Dictyostelium discoideum



Anti-14-3-3 sigma antibody - ab77187 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-14-3-3 sigma antibody (ab77187) at 0.1 µg/ml + human skin lysate in RIPA buffer at 35 µg

Anti-Actin antibody [2Q1055] - ab14128 from Abcam

Prices $392.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2Q1055
ab14128 staining Actin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab14128 at 5μg/ml and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an Goat anti-Mouse Alexa Fluor 488 secondary (ab150117) at 2 μg/ml (shown in green) and Goat anti-Rabbit Alexa Fluor 594 secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Anti-Actin antibody [ACTN05] (Biotin) - ab79667 from Abcam

Prices $372.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone ACTN05

Anti-Actin antibody [ACTN05 (C4)] - ab3280 from Abcam

Prices $404.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone ACTN05 (C4)
All lanes : Anti-Actin antibody [ACTN05 (C4)] (ab3280)Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 7 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell LysateLane 8 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell LysateLane 9 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Actin antibody [ACTN05; same as C4] - ab76548 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ACTN05; same as C4
ab76548 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab76548 at 10µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-mouse DyLight® 488 (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol fixed (100%, 5min) Hek293, HepG2, and MCF-7 cells at 10ug/ml.

Anti-alpha 1 Antichymotrypsin antibody - ab54693 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Standard Curve for alpha 1 Antichymotrypsin (Analyte: alpha 1 Antichymotrypsin protein (Human) (ab80517) ); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to alpha 1 Antichymotrypsin (ab54693) at 5µg/ml and Detector Antibody Rabbit polyclonal to alpha 1 Antichymotrypsin (ab9374) at 0.5µg/ml.

Anti-Calmodulin antibody [2D1] - ab2860 from Abcam

Prices $392.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 2D1
Flow cytometry analysis of Calmodulin showing positive staining in the cytoplasm of PC12 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant and adding 90% methanol followed by incubation for 10 minutes at room temperature. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2860 at 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Anti-CAPZB antibody - N-terminal - ab174368 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

Anti-Histone H3 (di methyl K36) antibody - ChIP Grade - ab9049 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab9049 staining Histone H3 (di methyl K36) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9049, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KDM2B antibody - ab5199 from Abcam

Prices $387.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-KDM2B antibody (ab5199) + HeLa whole cell lysatesSecondaryHRP-conjugated donkey anti-goat secondary antibody (chemicon international)

Anti-PPP2CB antibody - ab32673 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Calmodulin Antibody - LS-C77562 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-Calmodulin Antibody - LS-C77563 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-Calmodulin Antibody (clone 2D1) - LS-C84819 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 2D1

Anti-Calmodulin Antibody (clone 6D4) - LS-C84818 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 6D4

Anti-Calmodulin Antibody (clone CM-16, Magnetic beads) - LS-C171064 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone CM-16

Anti-Calmodulin Antibody (clone CM-16, Sepharose) - LS-C171065 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone CM-16

Anti-Calmodulin Antibody (clone CM-16) - LS-C171063 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone CM-16

Monoclonal Anti-Calmodulin antibody produced in mouse - C3545 from Sigma-Aldrich

Prices $268.50
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 6D4

Monoclonal Anti-Calmodulin antibody produced in mouse - C7055 from Sigma-Aldrich

Prices $359.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 2D1+6D4+1F11

Monoclonal Anti-Calmodulin antibody produced in mouse - C0931 from Sigma-Aldrich

Prices $287.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 1F11
<B>Immunoblotting</B><BR/>Reactivities of Anti-Calmodulin monoclonal antibodies with proteolytic fragements of <I>Dictyostelium</I> calmodulin. Purified <I>Dictyostelium</I> calmodulin (100 μg) was partially digested with clostripain. The proteolytic products were resolved by SDS-PAGE and transferred to a PVDF membrane for 3 hours. The primary antibodies were clone 1F11 (<B>Cat. No. C0931</B>), clone 6D4 (<B>Cat. No. C3545</B>) and clone 2D1 (<B>Cat. No. C6167</B>). <BR/>From Hulen, D., et al., Cell Motil. Cytoskel., <B>18</B>, 113 (1991). Courtesy of Dr. J. L. Salisbury, Mayo<SUP>®</SUP> Clinic, Rochester, MN.

Goat anti-Human 14-3-3 sigma polyclonal antibody - DPABH-17762 from Creative Diagnostics

Host Goat
Clonality Polyclonal

Mouse anti-Mouse Actin Monoclonal antibody [clone BDUO16], Biotin-Conjugated - DCABH-9919 from Creative Diagnostics

Host Mouse
Clonality Monoclonal
Clone BDUO16

Rat anti-Yeast Tubulin alpha Monoclonal antibody [clone ZM2/3], DyLight® 680-Conjugated - DCABY-757 from Creative Diagnostics

Host Rat
Clonality Monoclonal
Clone ZM2/3

Rat anti-Yeast Tubulin alpha Monoclonal antibody [clone ZM2/3], DyLight® 800-Conjugated - DCABY-758 from Creative Diagnostics

Host Rat
Clonality Monoclonal
Clone ZM2/3
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