Rice Oryza sativa



Anti-alpha COP I antibody - ab2913 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF of COP1 in Hela Cells

Anti-Beta Glucuronidase (GUS) antibody [27] - ab188492 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 27
All lanes : Anti-Beta Glucuronidase (GUS) antibody [27] (ab188492) at 1/1000 dilutionLane 1 : CK (TP309)Lane 2 : Transgenic rice protein ILane 3 : Transgenic rice protein IILane 4 : GUS fusion protein

Anti-beta Tubulin antibody - ab140698 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney (glomeruli) tissue labelling beta Tubulin with ab140698 at 10 µg/ml.

Anti-Histone H3 (acetyl K14) antibody - ChIP Grade - ab46984 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (acetyl K14) antibody - ChIP Grade (ab46984) at 1/1000 dilutionLane 1 : HeLa cells, no treatmentLane 2 : HeLa cells treated with sodium butyrateLysates/proteins at 10 µg per lane.

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade - ab4729 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab4729 staining Histone H3 (acetyl K27) in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4729, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H3 (acetyl K9) antibody [AH3-120] - ChIP Grade - ab12179 from Abcam

Prices $403.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone AH3-120
IHC image of ab12179 staining Histone H3 (acetyl K9) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12179, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H3 (acetyl K9) antibody - ChIP Grade - ab10812 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab10812 staining histone H3 (acetyl K9) in A549 cells treated with scriptaid (ab120883), by ICC/IF. Increase in histone H3 (acetyl K9) expression correlates with increased concentration of scriptaid, as described in literature.The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120883 (scriptaid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab10812 (0.1 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A anti-rabbit DyLight 488 secondary antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade - ab32356 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y47
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µl of ab32356 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade - ab1220 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 1220
Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220) at 1 µg/ml + HeLa (Human epitherlial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat polyclonal to Mouse IgG-H&L- Pre-Adsorbed (HRP) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (di methyl K9) antibody [mAbcam 62168] (HRP) - ab62168 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam 62168
All lanes : Anti-Histone H3 (di methyl K9) antibody [mAbcam 62168] (HRP) (ab62168) at 1/20000 dilutionLane 1 : Calf Thymus Histone Preparation Nuclear Lysate Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (unmodified ) peptide (ab2903) at 2.5 µg/mlLane 3 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K4) peptide (ab1340) at 2.5 µg/mlLane 4 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K4) peptide (ab7768) at 2.5 µg/mlLane 5 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K4) peptide (ab1342) at 2.5 µg/mlLane 6 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 2.5 µg/mlLane 7 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (di methyl K9) peptide (ab1772) at 2.5 µg/mlLane 8 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (tri methyl K9) peptide (ab1

Anti-Histone H3 (di+tri methyl K4) antibody [mAbcam 6000] - ChIP Grade - ab6000 from Abcam

Prices $404.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 6000
Immunofluorescent imaging of human cells (U2OS) with ab6000 reveals a diffuse background nuclear staining corresponding to global dimethylation of K4, with multiple foci of brighter staining.  The complete lack of nucleolar or cytoplasmic staining background confirms the high specificity of this antibody in this application.    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody Alexa 488 for 30 minutes.  All blocking and incubation steps carried out at 37 degrees.Due to the size constraints for images on our website, unfortunately we are unable to show a higher quality image.

Anti-Histone H3 (phospho T45) antibody - ab26127 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho T45) antibody (ab26127) at 1 µgLane 1 : HeLa treated with Calyculin A for 45min (0nM, untreated)Lane 2 : HeLa treated with Calyculin A for 45min (20nM)Lane 3 : HeLa treated with Calyculin A for 45min (20nM) with Human Histone H3 (phospho T45) peptide (ab26307) at 1 µgLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade - ab6002 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 6002
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 5µg of  ab6002 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.     

Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade - ab9050 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab9050 staining Histone H3 (tri-methyl K36) in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab9050 at 0.1µg/ml and ab7291 (anti beta-Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat anti -rabbit AlexaFluor®488 secondary (ab150081) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] (Alexa Fluor® 647) - ab196703 from Abcam

Prices $425.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mAbcam1012
ab196703 staining Histone H3 (tri methyl K4) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196703 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade - ab1012 from Abcam

Prices $394.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam1012
SK-N-SK cells were fixed in 4% paraformaldehdye, permeabilized in 0.5% Triton X-100 and incubated with ab1012 (1/100). The antibody clearly stains the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade - ab8580 from Abcam

Prices $404.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
IHC image of ab8580 staining Histone H3 (tri methyl K4) in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8580, 1/500 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade - ab1791 from Abcam

Prices $404.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 3 : Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr)Lane 4 : S.cerevisiae (Y190) Whole Cell Lysate Lane 5 : S.pombe Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade - ab51997 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1000Y
IHC image of ab51997 staining Histone H4 (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51997, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Lipooxygenase 2 antibody - ab82534 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
All lanes : Anti-Lipooxygenase 2 antibody (ab82534) at 1/1000 dilutionLane 1 : Recombinant proteinLane 2 : Molecular weight markers

Anti-Mre11 antibody - ab168817 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach linitis plastica tissue labelling Mre11 with ab168817 at 1/1000 (1µg/ml). Detection: DAB.

Anti-psbD antibody - ab65392 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-psbD antibody (ab65392) at 1/2000 dilutionLane 1 : Spinach thylakoidsLane 2 : Arabidopsis thylakoids

Anti-PSTAIR antibody [IL-16] - ab82217 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone IL-16

Anti-RNA polymerase II CTD repeat YSPTSPS antibody [8WG16] - ChIP Grade - ab817 from Abcam

Prices $401.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 8WG16
Various regions across the Actin2/7 loci were tested for the presence of RNA polymerase II CTD repeat YSPTSPS. A nuclear lysate from Arabidopsis thaliana seedlings was crosslinked using 1% formaldehyde for 30 seconds.  The ChIP was performed with 0.1 µg of ab817 per µg of chromatin; incubated together for 16 hours at 4°C.  The immunoprecipitated DNA was quantified by Real-Time PCR.  The bottom panel indicates the positive (ab817) and negative controls (no antibody) at region B3. See Abreview

Anti-TST antibody - ab60128 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-TST antibody (ab60128) at 1 µg/ml + Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/ 50,000 - 1/ 100,000 dilution.
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