Base excision repair



Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 9148 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D64E10
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with staurosporine #9953 (1 μM for 3 hours, right), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 6894 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D64E10
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with staurosporine #9953 (right) using Cleaved-PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red) and α-Tubulin (DM1A) Mouse mAb #3873 (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (PE Conjugate) - 8978 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D64E10
Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (PE Conjugate).

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 6987 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D64E10
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with staurosporine #9953 (1 μM for 3 hours, right), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #3623 (green).

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated) - 9185 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D64E10
Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr) using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated).

Cleaved PARP (Asp214) Antibody (Human Specific) (Fluorescein Conjugate) - 9547 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb - 5625 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D64E10
Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hr), Jurkat cells, untreated or etoposide-treated (25 μM, overnight), and THP-1 cells, untreated or cycloheximide-treated (CHX, 10 μg/ml, overnight) followed by treatment with TNF-α #8902 (20 ng/ml, 4 hr), using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (upper), or total PARP Antibody #9542 (lower).

Cleaved PARP (Asp214) Antibody (Human Specific) - 9541 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) Antibody (Human Specific).

Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) - 9546 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 200 µl (40 western blots), 600 µl (120 western blots)
Host Mouse
Clonality Monoclonal
Clone 19F4
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific).

PARP (46D11) Rabbit mAb - 9532 from Cell Signaling Technology

Prices $99.00, $261.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 46D11
Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb.

Cleaved PARP (Asp214) Antibody (Rat Specific) - 9545 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of C6 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Rat Specific).

PARP Antibody - 9542 from Cell Signaling Technology

Prices $99.00, $246.00, $596.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated (25 µM), using PARP Antibody.

cleaved PARP-1 (194C1439) - sc-56196 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/ 0.5 ml
Host Mouse
Clonality Monoclonal
PARP-1 (194C1439): sc-56196. Western blot analysis of PARP-1 expression in IMR-32 (A), Jurkat (B) and HeLa (C) whole cell lysates.

PARP-1 (5A5) - sc-56197 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PARP-1 (5A5): sc-56197. Western blot analysis of PARP-1 expression in Ramos (A), Jurkat (B), HeLa (C) and CCRF-CEM (D) nuclear extracts and Daudi (E) and Raji (F) whole cell lysates.

PARP-1 (A-20) - sc-1562 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PARP expression in Jurkat whole cell lysates (A,B). Antibodies tested include PARP-1 (N-20): sc-1561 (A) and PARP-1 (A-20): sc-1562 (B).

PARP-1 (B-10) - sc-74470 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PARP-1 CRISPR/Cas9 KO Plasmid (h): sc-400046. Western blot analysis of human PARP-1 expression in empty vector control (A) and PARP-1 CRISPR/Cas9 KO Plasmid transfected (B) HeLa whole cell lysates. Blot probed with PARP-1 (B-10): sc-74470. α-actinin (H-2): sc-17829 used as specificity and loading control.

PARP-1 (D-1) - sc-365315 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PARP-1 (D-1): sc-365315. Western blot analysis of PARP-1 expression in NIH/3T3 nuclear extract.

PARP-1 (E-8) - sc-74469 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PARP-1 (E-8): sc-74469. Western blot analysis of PARP-1 expression in non-transfected 293T: sc-117752 (A), mouse PARP-1 transfected 293T: sc-122382 (B) and Jurkat (C) whole cell lysates.

PARP-1 (F-2) - sc-8007 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PARP-1 (F-2): sc-8007. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lymphoma showing nuclear localization of PARP-1.

PARP-1 (H-300) - sc-25780 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PARP-1 (H-300): sc-25780. Western blot analysis of PARP-1 expression in Jurkat (A) and HeLa + etoposide (B) whole cell lysates.

PARP-1 (N-20) - sc-1561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PARP expression in Jurkat whole cell lysates (A,B). Antibodies tested include PARP-1 (N-20): sc-1561 (A) and PARP-1 (A-20): sc-1562 (B).

PARP-1 (H-250) - sc-7150 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PARP-1 (H-250): sc-7150. Western blot analysis of PARP-1 expression in Jurkat whole cell lysate.

PARP-1 (C2-10) - sc-53643 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µl ascites
Host Mouse
Clonality Monoclonal
PARP-1 (C2-10): sc-53643. Western blot analysis of PARP-1 expression in Ramos (A), Jurkat (B), HeLa (C) and CCRF-CEM (D) nuclear extracts and Daudi (E) and Raji (F) whole cell lysates.

PARP-1 (42) - sc-136208 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
PARP-1 (42): sc-136208. Immunofluorescence staining of BC3H1 cells showing nuclear localization.

Anti-Cleaved PARP antibody - ab4830 from Abcam

Prices $392.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Proteins from cell extracts were resolved by SDS-PAGE on a 4-20% Tris glycine gel and were transferred to PVDF membrane. Membranes were incubated with either 1 µg/mL anti-PARP (pan) antibody or anti PARP cleavage site (214/215) specific antibody at 1 µg/mL. After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that the anti-PARP cleavage site specific antibody only recognizes the 85 kDa fragment of PARP in apoptotic cells (lane 3) and does not react with full length PARP (lane 1). The PARP (pan) antibody confirms that nonapoptotic cells express full length PARP of 116 kDa (lane 2) and which is cleaved when apoptosis is induced (lane 4).
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