Recombinant Human AGAP1 293 Cell Lysate
Cat.No. : | AGAP1-8984HCL |
- Specification
- Gene Information
- Related Products
- Download
Description : | Antigen standard for ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (AGAP1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | AGAP1 ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 [ Homo sapiens ] |
Official Symbol : | AGAP1 |
Synonyms : | AGAP1; ArfGAP with GTPase domain, ankyrin repeat and PH domain 1; centaurin, gamma 2 , CENTG2; arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1; GGAP1; KIAA1099; centaurin-gamma-2; centaurin, gamma 2; GTP-binding and GTPase-activating protein 1; Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 1; AGAP-1; CENTG2; cnt-g2; MGC71657; |
Gene ID : | 116987 |
mRNA Refseq : | NM_001037131 |
Protein Refseq : | NP_001032208 |
MIM : | 608651 |
UniProt ID : | Q9UPQ3 |
Chromosome Location : | 2q37 |
Pathway : | Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; |
Function : | ARF GTPase activator activity; GTP binding; metal ion binding; nucleotide binding; zinc ion binding; |
Products Types
◆ Recombinant Protein | ||
AGAP1-94R | Recombinant Rhesus Macaque AGAP1 Protein, His (Fc)-Avi-tagged | +Inquiry |
AGAP1-0007H | Recombinant Human AGAP1 Protein, GST-Tagged | +Inquiry |
Agap1-1550M | Recombinant Mouse Agap1 Protein, Myc/DDK-tagged | +Inquiry |
AGAP1-536H | Recombinant Human AGAP1 Protein, MYC/DDK-tagged | +Inquiry |
AGAP1-266R | Recombinant Rhesus monkey AGAP1 Protein, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (20)
Ask a questionAGAP1 helps in the inactivation of Arf1 by accelerating its GTP hydrolysis, which is crucial for controlling vesicle trafficking between different cellular compartments.
Currently, there are no widely known drugs specifically targeting AGAP1, but research in this area could have therapeutic implications.
AGAP1 (Arf-GAP with GTPase, Ankyrin repeat, and PH domain 1) is a protein involved in regulating cellular processes like vesicle trafficking and membrane dynamics.
AGAP1 mutations have been associated with certain neurological conditions, potentially affecting neuronal development and function.
AGAP1 is one of several Arf-GAP proteins, each with distinct roles in cellular processes and vesicle trafficking pathways.
AGAP1 interacts with certain signaling molecules and receptors, influencing the transduction of signals within the cell.
The ankyrin repeat domain likely enables AGAP1 to interact with other proteins, contributing to its functional versatility.
AGAP1 has been linked to certain neurological disorders and cancers, but its precise roles in these contexts are still being investigated.
Yes, researchers often use techniques like gene knockdown or overexpression to study AGAP1's effects on cellular processes.
AGAP1 functions as a GTPase-activating protein (GAP) for the small GTPase Arf1, contributing to the regulation of membrane traffic and cellular signaling.
Yes, AGAP1's activity can be influenced by factors like cell stress, changes in membrane composition, and intracellular signaling.
AGAP1's functions are critical for maintaining proper cellular organization, membrane trafficking, and signal transduction, making it an important player in overall cell health.
AGAP1 helps regulate the formation and trafficking of vesicles between different cellular compartments, aiding in the proper distribution of cellular components.
AGAP1 interacts with Arf1, as well as other proteins involved in vesicle trafficking and membrane dynamics.
AGAP1 contains ankyrin repeat and PH (pleckstrin homology) domains, which are involved in protein-protein and protein-lipid interactions, respectively.
AGAP1 is found within cells, specifically in the cytoplasm and associated with membranes.
AGAP1 is expressed in various cell types, but its levels may vary depending on the tissue and cell context.
AGAP1 participates in processes like endocytosis, exocytosis, and maintenance of cellular membrane integrity.
AGAP1 stands for Arf-GAP with GTPase, Ankyrin repeat, and PH domain 1.
AGAP1's activity can be modulated by post-translational modifications, interactions with other proteins, and its localization within the cell.
Customer Reviews (5)
Write a reviewProtein's purity was evident in reproducible gel electrophoresis bands.
The product’s cost-effectiveness and clear instructions facilitated seamless incorporation into our work.
The insights gained from the protein product justified its cost, offering value for money.
It facilitated efficient DNA binding in all tested experimental conditions.
Despite its cost, the product’s contributions to our research goals rendered it an invaluable asset.
Ask a Question for All AGAP1 Products
Required fields are marked with *
My Review for All AGAP1 Products
Required fields are marked with *
Inquiry Basket