Recombinant Human ALPP 293 Cell Lysate
| Cat.No. : | ALPP-8893HCL |
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| Description : | Antigen standard for alkaline phosphatase, placental (Regan isozyme) (ALPP) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
| Source : | HEK 293 cells |
| Species : | Human |
| Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
| Size : | 0.1 mg |
| Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
| Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
| Gene Name : | ALPP alkaline phosphatase, placental [ Homo sapiens ] |
| Official Symbol : | ALPP |
| Synonyms : | ALPP; alkaline phosphatase, placental; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; Regan isozyme; PLAP-1; glycerophosphatase; alkaline phosphomonoesterase; placental alkaline phosphatase 1; alkaline phosphatase Regan isozyme; ALP; PALP; PLAP; FLJ61142; |
| Gene ID : | 250 |
| mRNA Refseq : | NM_001632 |
| Protein Refseq : | NP_001623 |
| MIM : | 171800 |
| UniProt ID : | P05187 |
| Chromosome Location : | 2q37.1 |
| Pathway : | Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; |
| Function : | alkaline phosphatase activity; alkaline phosphatase activity; hydrolase activity; metal ion binding; |
Products Types
| ◆ Recombinant Protein | ||
| ALPP-124H | Recombinant Human ALPP Protein, His-tagged | +Inquiry |
| ALPP-125H | Recombinant Human ALPP Protein, His-tagged | +Inquiry |
| ALPP-2558H | Recombinant Human ALPP protein(31-500 aa), C-His-tagged | +Inquiry |
| ALPP-219H | Recombinant Human ALPP protein(Ile23-Asp506), His-tagged | +Inquiry |
| ALPP-302H | Recombinant Human ALPP Protein, His tagged | +Inquiry |
| ◆ Native Protein | ||
| ALPP-8005H | Native Human Placental Alkaline Phosphatase | +Inquiry |
| ALPP-8347H | Native Human ALPP | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionALPP may serve as a diagnostic biomarker for specific diseases, allowing for disease detection and monitoring, and its targeting could hold therapeutic potential in certain pathological conditions.
ALPP participates in the metabolism and turnover of its substrate(s), including the hydrolysis of phosphoester bonds and the regulation of cellular phosphate homeostasis.
ALPP regulates downstream signaling pathways and molecular interactions involved in cellular processes like phosphate metabolism, intracellular signaling, and extracellular matrix remodeling.
ALPP gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in cellular homeostasis and disease processes.
ALPP is localized to specific cellular compartments, such as the plasma membrane, endoplasmic reticulum, or lysosomes, and its trafficking may be regulated by sorting signals and intracellular transport machinery.
ALPP activity can be modulated by factors such as pH, calcium, and inhibitors, which can affect its catalytic efficiency and cellular functions.
ALPP deficiency or dysregulation is associated with various diseases, including metabolic disorders, bone diseases, and certain cancers, highlighting its pathological implications.
Manipulating ALPP activity and function could offer strategies to modulate cellular processes and disease outcomes, potentially leading to therapeutic interventions and improved patient outcomes.
ALPP expression and activity exhibit dynamic changes during development, cellular differentiation, and disease progression, reflecting its roles in different physiological and pathological contexts.
ALPP shows a tissue-specific expression pattern, with higher levels in tissues such as liver, bone, and kidney, and lower levels in other organs.
Customer Reviews (3)
Write a reviewHigh-throughput screening compatibility.
Consistent protein expression analysis.
Precise protein quantification in ELISA.
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