Recombinant Human AMBRA1 293 Cell Lysate
| Cat.No. : | AMBRA1-8887HCL |
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| Description : | Antigen standard for autophagy/beclin-1 regulator 1 (AMBRA1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
| Source : | HEK 293 cells |
| Species : | Human |
| Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
| Size : | 0.1 mg |
| Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
| Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
| Gene Name : | AMBRA1 autophagy/beclin-1 regulator 1 [ Homo sapiens ] |
| Official Symbol : | AMBRA1 |
| Synonyms : | AMBRA1; autophagy/beclin-1 regulator 1; activating molecule in BECN1-regulated autophagy protein 1; DCAF3; DDB1 and CUL4 associated factor 3; FLJ20294; KIAA1736; WD repeat domain 94; WDR94; activating molecule in beclin-1-regulated autophagy; MGC33725; |
| Gene ID : | 55626 |
| mRNA Refseq : | NM_017749 |
| Protein Refseq : | NP_060219 |
| MIM : | 611359 |
| UniProt ID : | Q9C0C7 |
| Chromosome Location : | 11p11.2 |
| Pathway : | Senescence and Autophagy, organism-specific biosystem; |
Products Types
| ◆ Recombinant Protein | ||
| AMBRA1-501M | Recombinant Mouse AMBRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
| AMBRA1-01H | Active Recombinant Human AMBRA1 Protein, Myc/DDK-tagged | +Inquiry |
| Ambra1-1608M | Recombinant Mouse Ambra1 Protein, Myc/DDK-tagged | +Inquiry |
| AMBRA1-332H | Recombinant Human AMBRA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
| AMBRA1-9609H | Recombinant Human AMBRA1 protein, His-tagged | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAMBRA1 undergoes post-translational modifications, including phosphorylation and ubiquitination, which regulate its interaction with other autophagy-related proteins and modulate its function in autophagy.
AMBRA1 participates in selective autophagy processes, such as mitophagy (selective clearance of damaged mitochondria) and aggrephagy (clearance of protein aggregates), contributing to cellular quality control mechanisms.
AMBRA1 plays a critical role in autophagy regulation by promoting autophagosome formation, facilitating cargo recognition and degradation, and coordinating the activity of other autophagy-related proteins.
AMBRA1 facilitates the clearance of protein aggregates and damaged organelles through autophagy, preventing their accumulation and mitigating cellular damage.
AMBRA1 expression is induced during autophagy by various signaling pathways, including nutrient deprivation, cellular stress, and growth factor signaling.
In addition to its role in autophagy, AMBRA1 interacts with other cellular processes, such as apoptosis and cell proliferation, forming intricate crosstalk that influences cellular fate and homeostasis.
AMBRA1 interacts with Beclin 1, ULK1, and ATG proteins through protein-protein interactions, forming complexes that regulate autophagy initiation and autophagosome formation.
Depletion of AMBRA1 leads to impaired autophagy activity, resulting in the accumulation of damaged proteins and organelles and compromised cellular homeostasis.
AMBRA1 promotes autophagosome formation by regulating the nucleation and elongation steps, ensuring proper assembly of the autophagy machinery and cargo sequestration.
The activity of AMBRA1 in autophagy is modulated by upstream signaling pathways, including mTORC1, AMPK, and MAPK pathways, which integrate nutrient availability and cellular stresses.
Customer Reviews (3)
Write a reviewEasy integration into multi-omics workflows for comprehensive analysis.
Wide dynamic range for accurate quantification of protein changes.
Low false-positive rate in target protein identification.
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