Recombinant Human ANAPC2 293 Cell Lysate
Cat.No. : | ANAPC2-8865HCL |
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Description : | Antigen standard for anaphase promoting complex subunit 2 (ANAPC2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | ANAPC2 anaphase promoting complex subunit 2 [ Homo sapiens ] |
Official Symbol : | ANAPC2 |
Synonyms : | ANAPC2; anaphase promoting complex subunit 2; anaphase-promoting complex subunit 2; APC2; KIAA1406; cyclosome subunit 2; RP11-350O14.5; |
Gene ID : | 29882 |
mRNA Refseq : | NM_013366 |
Protein Refseq : | NP_037498 |
MIM : | 606946 |
UniProt ID : | Q9UJX6 |
Chromosome Location : | 9q34.3 |
Pathway : | APC/C complex, organism-specific biosystem; APC/C complex, conserved biosystem; APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; |
Function : | ubiquitin protein ligase binding; ubiquitin-protein ligase activity; |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (20)
Ask a questionAs ANAPC2 plays a crucial role in cell cycle regulation, it has the potential to be targeted for therapeutic interventions. However, at present, there are no specific therapies that directly target ANAPC2. Research is ongoing to explore the potential of ANAPC2 as a therapeutic target for cancer and other diseases.
While the primary function of ANAPC2 is in cell cycle regulation, it indirectly influences DNA repair processes by ensuring proper cell cycle control and accurate chromosome segregation during mitosis. By preventing the accumulation of damaged DNA, ANAPC2 indirectly contributes to maintaining genome stability and integrity.
ANAPC2 is critical for cell viability. Studies using cell lines with ANAPC2 knockdown or knockout have shown severe defects in cell cycle progression, leading to cell cycle arrest and cell death. This highlights the essential role of ANAPC2 in cellular processes.
Studies have shown that dysregulation or mutations in ANAPC2 can have significant impacts on cell cycle control and genomic stability, which can contribute to the development of various diseases. For example, ANAPC2 mutations have been associated with colorectal cancer and hepatocellular carcinoma.
Loss-of-function mutations in ANAPC2 can have severe consequences on cell cycle regulation and genomic stability. Studies in animal models have shown that ANAPC2 deficiency leads to embryonic lethality due to disrupted cell proliferation and improper organ development. In human cells, ANAPC2 mutations can contribute to various diseases, including cancer, by disrupting the normal control mechanisms of the cell cycle.
ANAPC2 can undergo post-translational modifications, such as phosphorylation and acetylation, which can potentially regulate its stability and activity. These modifications can be critical for proper cell cycle control.
The dysregulation of the cell cycle machinery, including ANAPC2, is a hallmark of cancer. Targeting components of the cell cycle, including ANAPC2, is an area of active research for developing novel cancer therapies. Inhibitors of the APC/C complex or ANAPC2-specific molecules may hold potential for selectively targeting cancer cells and disrupting their cell cycle progression.
ANAPC2 is a subunit of the APC/C complex, which is one of the key regulators of the cell cycle progression. APC/C interacts with various proteins and pathways involved in cell cycle control, including cyclins, cyclin-dependent kinases (CDKs), E3 ligases, and checkpoint regulators. ANAPC2 interacts with other subunits of the APC/C complex to facilitate protein degradation and ensure proper cell cycle transitions.
ANAPC2 forms tight complexes with other subunits of the APC/C complex, including ANAPC3, ANAPC7, and ANAPC10. These interactions are necessary for the stability and activity of the complex.
ANAPC2 expression levels or genetic variations in ANAPC2 have been investigated as potential biomarkers for certain diseases, particularly cancer. Changes in ANAPC2 expression or mutations may be indicative of altered cell cycle regulation and genomic instability. However, more research is needed to establish ANAPC2 as a reliable biomarker.
ANAPC2 indirectly contributes to maintaining genome stability by ensuring proper cell cycle control and regulation of chromosome segregation during mitosis. By targeting proteins involved in DNA replication, repair, and cell division for degradation, ANAPC2 helps prevent errors in genome replication and segregation.
While ANAPC2's primary role is in cell cycle regulation, dysregulation of the APC/C complex, including ANAPC2, can have broader implications in other cellular pathways. For example, defects in APC/C function can lead to chromosomal instability, aberrant DNA repair, and altered cellular signaling. Understanding these interactions can provide insights into the role of ANAPC2 in various cellular processes.
While ANAPC2 is primarily studied in the context of cancer, its dysregulation and role in cell cycle control may have implications in other diseases. Further research is needed to determine if ANAPC2 can be targeted for therapeutic interventions in diseases like neurodegenerative disorders, autoimmune diseases, or developmental disorders.
There is limited research suggesting potential links between ANAPC2 and neurodegenerative disorders. ANAPC2 has been identified in genetic studies in relation to amyotrophic lateral sclerosis (ALS) and Parkinson's disease, but these associations require further investigation to clarify the exact role of ANAPC2 in neurodegeneration.
ANAPC2 mutations have the potential to impact drug response in cancer treatment. Dysregulation of the cell cycle machinery, including ANAPC2, can lead to uncontrolled cell growth and resistance to chemotherapy agents that target proliferating cells. Further research is needed to fully understand how ANAPC2 mutations may contribute to drug resistance and potential strategies to overcome it.
Genetic variations in ANAPC2 have been identified through genome sequencing studies. These variations can include single nucleotide polymorphisms (SNPs), insertions, deletions, or structural variations. Some of these genetic variations may have functional consequences and could be associated with disease susceptibility or progression.
ANAPC2 is essential for proper embryonic development. Studies in animal models have shown that loss of ANAPC2 function leads to embryonic lethality, indicating its critical role in early development. ANAPC2 is required for cell proliferation, differentiation, and normal organ formation during embryogenesis.
ANAPC2's direct interactions with the immune system are not well established. However, as ANAPC2 is involved in cell cycle regulation and genomic stability, dysregulation of ANAPC2 or APC/C complex function could potentially impact immune cell proliferation, differentiation, and response to infection or immunotherapy. Further studies are needed to understand the involvement of ANAPC2 in immune system regulation.
ANAPC2 is a large protein subunit composed of around 900 amino acids. It contains several functional domains, including an N-terminal TPR (tetratricopeptide repeat) domain involved in protein-protein interactions and a C-terminal domain responsible for the catalytic activity of the APC/C complex. The three-dimensional structure of ANAPC2 has been determined through research.
ANAPC2 primarily interacts with other subunits of the APC/C complex to carry out its functions in cell cycle regulation. However, dysregulation of the APC/C complex, including ANAPC2, can have broader implications in cellular pathways involved in DNA repair, chromosomal stability, and cell proliferation.
Customer Reviews (4)
Write a reviewthe manufacturer's commitment to scientific collaboration and knowledge sharing greatly enhances my research experience.
They ensure a consistent and reliable supply of ANAPC16 protein, minimizing any potential disruptions in my experimental workflow.
Their excellent technical support, commitment to scientific collaboration, and efficient supply chain management greatly contribute to the success and productivity of my research.
This collaborative approach empowers me to integrate cutting-edge knowledge, explore innovative techniques, and expand the impact of my research.
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