Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) Protein

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Catalogue No: abx065478
Price: US$246.50
(Size: 10 µg)

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Datasheet SDS
Human ATPase, H+ / K+ Exchanging Alpha Polypeptide (ATP4a) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).

Target ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
Origin Human
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P20648 (UniProt, ExPASy)
UniProt Secondary AC O00738
UniProt Entry Name ATP4A_HUMAN
KEGG hsa:495
String 9606.ENSP00000262623
Molecular Weight Calculated MW: 12.7 kDa
Observed MW (SDS-PAGE): 17 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Leu798-Gly896
Sequence LTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFTDY FTAMAQEGWF PLLCVG
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)


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