Active Native Human a-Macroglobulin
| Cat.No. : | a-Macroglobulin-535H |
| Product Overview : | Native Human a-Macroglobulin was purified from human plasma. |
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| Description : | alpha-2-Macroglobulin, also known as α2-macroglobulin and abbreviated as α2M and A2M, is a large plasma protein found in the blood. It is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. |
| Source : | human plasma |
| Species : | Human |
| Form : | Lyophilized from 0.02 M Tris, 0.13 M glycine, pH 8.0, and 0.08 M trehalose |
| Bio-activity : | This product is a preparation of the intact protein with proteolytic inhibitory activity. One mg of protein will inhibit a minimum of 10 mg of trypsin with an activity of 10,000 BAEE units/mg protein. |
| Molecular Mass : | mol wt ~720 kDa (four glycoprotein subunits) |
| Purity : | ≥98% (SDS-PAGE) |
| Applications : | Inhibits all classes of endoproteases by forming a complex with the protease. When the protease cleaves the macroglobulin sequence, the macroglobulin rearranges and traps the protease. |
| Notes : | Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg. |
| Storage : | The lyophilized powder is stable for at least 2 years when stored at -20 °C. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionAlpha-Macroglobulin interacts with various extracellular matrix components, including collagens, elastin, and glycosaminoglycans, influencing tissue remodeling, cell adhesion, and matrix homeostasis in different physiological and pathological contexts.
Targeting alpha-Macroglobulin may provide therapeutic opportunities to modulate protease activity in specific pathological conditions, either by enhancing its inhibitory function or developing small molecules that mimic its protease-trapping activity.
Alpha-Macroglobulin levels or activity may serve as a diagnostic marker for conditions associated with abnormal protease activity, such as cancer, cardiovascular diseases, or neurodegenerative disorders.
Alpha-Macroglobulin interacts with cell surface receptors, such as low-density lipoprotein receptor-related protein 1 (LRP1), to mediate endocytosis and downstream signaling events that regulate cell behavior and inflammation.
The structural features of alpha-Macroglobulin, such as its bait region and thioester domain, play critical roles in recognizing and trapping proteases, allowing for their subsequent clearance or degradation.
Alpha-Macroglobulin interacts with a wide range of proteases, including serine proteases, metalloproteases, and cysteine proteases, targeting specific cleavage sites within their catalytic domains.
Alpha-Macroglobulin is involved in modulating inflammation and immune responses by regulating the activity of proteases that are implicated in these processes, thereby influencing the activation and function of immune cells.
Alpha-Macroglobulin acts as a protease inhibitor by undergoing conformational changes upon protease binding, leading to the formation of a covalent complex that sequesters the protease and prevents its activity.
Alpha-Macroglobulin clearance occurs through receptor-mediated endocytosis, with LRP1 playing a major role in its internalization and subsequent trafficking to lysosomes for degradation.
Genetic variations or mutations in alpha-Macroglobulin can affect its protease inhibitory capacity, leading to altered protease regulation and potential associations with diseases such as familial hypercholesterolemia or Alzheimer's disease.
Customer Reviews (3)
Write a reviewMinimal sample consumption for precious samples.
Easy adaptation to high-throughput formats for large-scale experiments.
Accurate determination of protein stability under different conditions.
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