Recombinant Human Alcohol Dehydrogenase 1A (class I), Alpha Polypeptide, His-tagged
| Cat.No. : | ADH1A-1917H |
| Product Overview : | Recombinant humanADH1A protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques. |
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| Cat. No. : | ADH1A-1917H |
| Description : | ADH1Abelongs to the alcohol dehydrogenase family. ADH1A is a monomorphic andpredominant in fetal and infant livers, becoming less active in gestation andonly weakly active during adulthood. ADH1A plays a major role in ethanolmetabolism. With the coenzyme NAD, ADH catalyzes the reversible conversion oforganic alcohols to ketones or aldehydes. |
| Form : | Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl. |
| Molecular Weight : | 42 kDa(395aa) confirmed by MALDI-TOF |
| Purity : | > 90%by SDS-PAGE |
| Concentration : | 1 mg/ml(determined by Bradford assay) |
| Sequences of aminoacids : | MGSSHHHHHHSSGLVPRGSH MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAVGICGTD DHVVSGTMVTPLPVILGHEAAGIVESVGEGVTTVKPGDKV IPLAIPQCGK CRICKNPESN YCLKNDVSNP QGTLQDGTSR FTCRRKPIHH FLGISTFSQYTVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG LGGVGLSAIM GCKAAGAARIIAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTDGGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSMNPMLLL TGRTWKGAIL GGFKSKECVPKLVADFMAKK FSLDALITHV LPFEKINEGF DLLHSGKSIR TILMF |
| Storage : | Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| Pathways : | Biological oxida; Drug metabolism-cytochromeP450; Ethanol oxidation; Fatty Acid Omega Oxidation; Fatty acid metabo;Glycolysis / Gluconeogenesis; Metabolic pathways; Metabolism; Metabolism ofxenobiotics by cytochrome P450; Phase 1-Functionalization of compounds;Retinol metabolism; Tyrosine metabolism; Vitamin A and carotenoid metabolism |
| SDS-PAGE: |
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| Gene Name : | ADH1A alcohol dehydrogenase 1A(class I), alpha polypeptide [ Homo sapiens ] |
| Official Symbol : | ADH1A |
| Synonyms : | ADH1A; alcohol dehydrogenase 1A (class I),alpha polypeptide; ADH1; alcohol dehydrogenase 1A; ADH, alphasubunit; aldehyde reductase; alcohol dehydrogenase subunit alpha; alcoholdehydrogenase 1 (class I), alpha polypeptide; EC 1.1.1.1; Alcohol dehydrogenase 1A |
| Gene ID : | 124 |
| mRNA Refseq : | NM_000667 |
| Protein Refseq : | NP_000658 |
| MIM : | 103700 |
| UniProt ID : | P07327 |
| Chromosome Location : | 4q23 |
| Function : | alcohol dehydrogenase activity; metal ionbinding; nucleotide binding; oxidoreductase activity; protein binding; zincion binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe expression of ADH1A is regulated by specific mechanisms, including transcriptional and post-transcriptional regulation, which can respond to alcohol exposure or metabolic factors such as hormonal signaling.
ADH1A requires specific cofactors or coenzymes, such as NAD+ or NADH, for its activity, serving as electron carriers and modulators of ADH1A function.
ADH1A plays a significant role in alcohol metabolism, showing specific catalytic efficiency and substrate specificity for the conversion of alcohol to acetaldehyde.
ADH1A deficiency or knockout in experimental models leads to altered alcohol metabolism and related phenotypes, such as decreased alcohol clearance and increased sensitivity to alcohol-induced effects.
ADH1A exhibits a tissue-specific expression pattern, with higher expression levels in certain organs such as the liver, and its distribution may differ from other ADH isoforms, reflecting functional specialization.
Genetic variations or polymorphisms in the ADH1A gene have been associated with differences in alcohol metabolism and susceptibility to alcohol-related disorders, highlighting the impact of genotype on phenotype.
Pharmacological agents or environmental factors can modulate ADH1A expression or activity, potentially influencing alcohol metabolism and providing avenues for therapeutic interventions or personalized medicine.
ADH1A has the capacity to metabolize other substrates beyond alcohol, suggesting its involvement in broader metabolic pathways and potential metabolic interactions.
ADH1A expression or activity may vary among different species or populations, influenced by genetic and environmental factors, contributing to inter-individual or inter-species differences in alcohol metabolism and tolerance.
The structural features of ADH1A, such as its active site and binding domains, are critical for its enzymatic activity and substrate recognition, enabling efficient alcohol oxidation.
Customer Reviews (2)
Write a reviewExhibits remarkable enzymatic activity under a wide range of pH conditions.
Provides reliable and consistent results in protein folding and unfolding studies.
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