Recombinant Arabidopsis thaliana Peroxisome biogenesis protein 7 (PEX7)

Code CSB-YP895889DOA
MSDS
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Source Yeast
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Code CSB-EP895889DOA
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Source E.coli
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Code CSB-EP895889DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895889DOA
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Source Baculovirus
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Code CSB-MP895889DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEX7
Uniprot No.
Alternative Names
PEX7; At1g29260; F28N24.6Peroxisome biogenesis protein 7; Peroxin-7; AtPEX7; Peroxisomal targeting signal type 2 receptor; Pex7p
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-317
Target Protein Sequence
MPVFKAPFNG YSVKFSPFYE SRLAVATAQN FGILGNGRIH VLELAPGAPG VTESVSYDTA DAVYDVCWSE SHDSVLIAAI GDGSVKIYDT ALPPPSNPIR SFQEHAREVQ SVDYNPTRRD SFLTSSWDDT VKLWAMDRPA SVRTFKEHAY CVYQAVWNPK HGDVFASASG DCTLRIWDVR EPGSTMIIPA HDFEILSCDW NKYDDCILAT SSVDKTVKVW DVRSYRVPLA VLNGHGYAVR KVKFSPHRRS LIASCSYDMS VCLWDYMVED ALVGRYDHHT EFAVGIDMSV LVEGLMASTG WDELVYVWQQ GMDPRAS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Import receptor for peroxisomal-targeting signal two (PTS2). A receptor-cargo complex composed of PEX5, PEX7, a PTS1-containing protein and a PTS2-containing protein is targeted to peroxisomes during import.
Gene References into Functions
  1. Peroxisomal plant nitric oxide synthase (NOS) protein is imported by peroxisomal targeting signal type 2 (PTS2) in a process that depends on the cytosolic receptor PEX7 and calmodulin PMID: 24801177
  2. RabE1c binds PEX7 and facilitates PEX7 degradation in the presence of immobile GFP-PEX7 accumulated at the membrane PMID: 23297417
  3. results suggest that the targeting of PEX7 to peroxisomes requires four proteins: a PTS2-containing protein, PEX14, PEX13 and PEX12. PMID: 19594707
Subcellular Location
Cytoplasm. Peroxisome membrane; Peripheral membrane protein. Note=The loss of PEX12, PEX13 or PEX14 prevents the targeting of PEX7 to peroxisomes.
Protein Families
WD repeat peroxin-7 family
Tissue Specificity
Expressed in siliques and leaves, but barely detectable in flowers, stems and roots.
Database Links

KEGG: ath:AT1G29260

STRING: 3702.AT1G29260.1

UniGene: At.11271

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