Recombinant Mesocricetus auratus Cholesterol side-chain cleavage enzyme, mitochondrial (CYP11A1)

Code CSB-YP887646MRG
MSDS
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Source Yeast
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Code CSB-EP887646MRG
MSDS
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Source E.coli
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Code CSB-EP887646MRG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887646MRG
MSDS
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Source Baculovirus
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Code CSB-MP887646MRG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP11A1
Uniprot No.
Alternative Names
CYP11A1; CYP11ACholesterol side-chain cleavage enzyme; mitochondrial; EC 1.14.15.6; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
Species
Mesocricetus auratus (Golden hamster)
Expression Region
37-520
Target Protein Sequence
ISTN SPRPFNEIPS PGDNGWLNLY HFWRENGTHR IHYHHMQNFQ KYGPIYREKL GNKDSVYILD PEDAAQLFLS EGPYPERYLV PPWVAYHQYY KRPIGVLFKS SEAWKKDRLV LNQEVMAPEA IKNFVPLLEG VVQDFINVLH RRIKQQKSGN FSGDISDDLF RFAFESITSV VFGERLGMLE EIVDPESQRF IDAIYQMFHT SVPMLNLPPE LFRFFRTKTW KEHAAAWDMI FKKADDYTQT FYWDLRQKQE FSKYPGVLYS LLGGNKLPFK NIQANITEML AGGVDTTSMT LQWSLYEMAH NLKVQEMLRA EVLAARRQAQ GDMVKMVQLV PLLKASIKET LRLHPISVTV QRYLVDDLVL RNYRIPAKML VQVANYAMGR EPSFFPNPNK FDPTRWLEKS KNTTHFRYLS FGWGVRQCLG RRIAELEMTI FLINVLENFR IELQSLHDVG TKFNLILMPE KPILFNLQPL KKDLGTTTNR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin).
Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
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