Recombinant Arabidopsis thaliana Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase (PAT)

Code CSB-YP871074DOA
MSDS
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Source Yeast
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Code CSB-EP871074DOA
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Source E.coli
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Code CSB-EP871074DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871074DOA
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Source Baculovirus
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Code CSB-MP871074DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PAT
Uniprot No.
Alternative Names
PAT; AAT; MEE17; At2g22250; T26C19.9; Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase; AtAAT; AtPPA-AT; EC 2.6.1.1; EC 2.6.1.78; EC 2.6.1.79; Protein MATERNAL EFFECT EMBRYO ARREST 17
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
54-475
Target Protein Sequence
AMAKPND AETLSSSVDM SLSPRVQSLK PSKTMVITDL AATLVQSGVP VIRLAAGEPD FDTPKVVAEA GINAIREGFT RYTLNAGITE LREAICRKLK EENGLSYAPD QILVSNGAKQ SLLQAVLAVC SPGDEVIIPA PYWVSYTEQA RLADATPVVI PTKISNNFLL DPKDLESKLT EKSRLLILCS PSNPTGSVYP KSLLEEIARI IAKHPRLLVL SDEIYEHIIY APATHTSFAS LPDMYERTLT VNGFSKAFAM TGWRLGYLAG PKHIVAACSK LQGQVSSGAS SIAQKAGVAA LGLGKAGGET VAEMVKAYRE RRDFLVKSLG DIKGVKISEP QGAFYLFIDF SAYYGSEAEG FGLINDSSSL ALYFLDKFQV AMVPGDAFGD DSCIRISYAT SLDVLQAAVE KIRKALEPLR ATVSV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Prokaryotic-type aspartate aminotransferase. Has also a prenate transaminase activity. Involved in the aromatic amino acids biosynthesis pathway via the arogenate route. Required for the transamination of prephenate into arogenate. Required for early development of the embryo.
Gene References into Functions
  1. Data show that the purifies prephenate aminotransferase activity is housed by the prokaryotic-type plastidic aspartate aminotransferase (At2g22250). PMID: 20883697
Subcellular Location
Plastid, chloroplast.
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links

KEGG: ath:AT2G22250

STRING: 3702.AT2G22250.2

UniGene: At.14448

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