Recombinant Bovine PHD finger protein 10 (PHF10)

Code CSB-YP017898BO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP017898BO
MSDS
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Source E.coli
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Code CSB-EP017898BO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017898BO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017898BO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PHF10
Uniprot No.
Alternative Names
PHF10; BAF45A; PHD finger protein 10; BRG1-associated factor 45a; BAF45a
Species
Bos taurus (Bovine)
Expression Region
1-410
Target Protein Sequence
MLQEQVSEYL GVTSFKRKYP DLERRDLSHK EKLYLRELNV ITETQCTLGL TALRSDEVID LMIKEYPAKH AEYSVILQEK ERQRITDHYK EYSQMQQQNT QKVEASKVPE YIKKAAKKAA EFNSNLNRER MEERRAYFDL QTHVIQVPQG KYKVLPTERT KVSSYPVALI PGQFQEYYKR YSPDELRYLP LNTALYEPPL DPELPALESD GDSDDAEDGR GDEKGKSKGT SDSSSGNVSE GEGLPEGQEE PLPGRQRPRD KAAAPRKDAP KRSALSKAVP GHKPKVIPNA LCGICLKGKE SSRRGKAEPL VHCSQCDNSG HPSCLDMTME LVSMIKTYPW QCMECKTCII CGQPHHEEEM MFCDVCDRGY HTFCVGLGAI PSGRWICDCC QRAPPTPRKV GRRGKNSKEG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
Subcellular Location
Nucleus.
Protein Families
SAYP family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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