Recombinant Arabidopsis thaliana Cytochrome P450 90B1 (CYP90B1), partial

Code CSB-YP527715DOA
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Source Yeast
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Code CSB-EP527715DOA
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Source E.coli
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Code CSB-EP527715DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527715DOA
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Source Baculovirus
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Code CSB-MP527715DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP90B1
Uniprot No.
Alternative Names
CYP90B1; DWF4; At3g50660; T3A5.40Cytochrome P450 90B1; EC 1.14.-.-; Protein DWARF 4; Dwarf4; Steroid 22-alpha-hydroxylase
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the C22-alpha-hydroxylation step in brassinosteroids biosynthesis. Converts campestanol to 6-deoxocathasterone and 6-oxocampestanol to cathasterone.
Gene References into Functions
  1. TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana PMID: 26233893
  2. Overexpression of CYP724A1 in null dwf4-102 plants functionally complements their C-22 hydroxylase deficiency. PMID: 22196800
  3. TCP1 positively regulates BR biosynthesis via its direct regulation of the transcription of DWF4. PMID: 21822059
  4. DWF4 transcription plays a novel role in the brassinosteroid (BR)-auxin crosstalk associated with root elongation, in addition to its role in BR homeostasis. PMID: 21909364
  5. The binding of BZR1 (brassinazole-resistant 1 protein) to the DWF4 promoter is attenuated by treatment with auxin. PMID: 21284753
  6. Studies indicate that DWF4 gene encodes a 22-a hydroxylase (CYP90B1) that catalyzes the conversion of 6- Oxocampestanol/Campestanol to 6-Deoxocathasterone/Cathasterone. PMID: 21103336
  7. TCP1 positively regulates the expression of the key brassinosteroid biosynthetic gene, DWF4. PMID: 20435901
  8. Brassinosteroid negatively regulates jasmonate inhibition of root growth, and DWF4 is down-regulated by jasmonate. PMID: 19741050
  9. It is likely that the DWF4 promoter serves as a focal point in maintaining homeostasis of endogenous bioactive brassinosteroids pools in specific tissues of Arabidopsis. PMID: 16407451
  10. may explain the discrepancy between the in planta abundance of C27/C28/C29 sterols and C27/C28/C29 brassinosteroids PMID: 16460510
  11. Transcripts for dwf4 accumulated in the epidermis of young leaf primordia and targeted expression of DWF4 to a subset of these cells led to both restoration of differentiation of a specific group of leaf cells and restoration of wild-type leaf shape. PMID: 17908154

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Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Expressed in stems, leaves, shoots, and roots, with a higher expression in siliques and apical shoots.
Database Links

KEGG: ath:AT3G50660

STRING: 3702.AT3G50660.1

UniGene: At.26401

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