Recombinant Escherichia coli Multidrug efflux pump subunit AcrA (acrA)

Code CSB-YP360036ENV
MSDS
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Source Yeast
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Code CSB-EP360036ENV-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360036ENV
MSDS
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Source Baculovirus
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Code CSB-MP360036ENV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
acrA
Uniprot No.
Alternative Names
acrA; lir; mtcA; b0463; JW0452Multidrug efflux pump subunit AcrA; AcrAB-TolC multidrug efflux pump subunit AcrA; Acridine resistance protein A
Species
Escherichia coli (strain K12)
Expression Region
25-397
Target Protein Sequence
CDDKQA QQGGQQMPAV GVVTVKTEPL QITTELPGRT SAYRIAEVRP QVSGIILKRN FKEGSDIEAG VSLYQIDPAT YQATYDSAKG DLAKAQAAAN IAQLTVNRYQ KLLGTQYISK QEYDQALADA QQANAAVTAA KAAVETARIN LAYTKVTSPI SGRIGKSNVT EGALVQNGQA TALATVQQLD PIYVDVTQSS NDFLRLKQEL ANGTLKQENG KAKVSLITSD GIKFPQDGTL EFSDVTVDQT TGSITLRAIF PNPDHTLLPG MFVRARLEEG LNPNAILVPQ QGVTRTPRGD ATVLVVGADD KVETRPIVAS QAIGDKWLVT EGLKAGDRVV ISGLQKVRPG VQVKAQEVTA DNNQQAASGA QPEQSKS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
AcrA-AcrB-AcrZ-TolC is a drug efflux protein complex with broad substrate specificity that uses the proton motive force to export substrates. This subunit may act as an adapter protein that links AcrB and TolC stably together. It is elongated in shape, being long enough to span the periplasm.
Gene References into Functions
  1. The results underscore the importance of the periplasmic turn 1 of TolC in the functional assembly of the tripartite efflux complex and AcrA in transitioning TolC from its closed to open state. PMID: 20132445
  2. Our results indicate functional interactions between the AcrA -hairpin tip region and the TolC aperture tip region in the assembly of the functional tripartite efflux pump AcrAB-TolC. PMID: 20581201
  3. Data show that the AcrA-mediated suppression of mutant TolC phenotypes and stabilization of mutant TolC were dependent on AcrB, reflecting the existence of a functional complex between TolC and AcrAB in vivo. PMID: 15491355
  4. TolC-AcrA interaction demonstrated via cross-linking studies PMID: 15576805
  5. The observed pulsating behavior in inducible transcription of acrA is a systems property arising from the dependence of gene expression on multidrug resistance determinants. PMID: 16461398
  6. AcrS represses the gene expression of multidrug efflux genes, acrA and acrB PMID: 18567659
  7. Data suggest that recruitment of TolC by various MFPs AcrA, MacA, and EmrA is determined not only by kinetics of MFP-TolC interactions but also by oligomerization kinetics of MFPs and pH. PMID: 19805313

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Subcellular Location
Cell inner membrane; Lipid-anchor. Note=An unlipidated version of this protein (directed to the periplasm by the OmpA signal sequence) functions normally.
Protein Families
Membrane fusion protein (MFP) (TC 8.A.1) family
Database Links
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