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Recombinant Escherichia coli Secretion monitor (secM)

Recombinant Escherichia coli Secretion monitor (secM)

Code	CSB-YP351745ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP351745ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP351745ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP351745ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP351745ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

secM

Uniprot No.

P62395

Alternative Names

secM; srrA; yacA; b0097; JW5007; Secretion monitor

Species

Escherichia coli (strain K12)

Expression Region

38-170

Target Protein Sequence

AEP NAPAKATTRN HEPSAKVNFG QLALLEANTR RPNSNYSVDY WHQHAIRTVI RHLSFAMAPQ TLPVAEESLP LQAQHLALLD TLSALLTQEG TPSEKGYRID YAHFTPQAKF STPVWISQAQ GIRAGPQRLT

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Regulates secA expression by translational coupling of the secM secA operon. Ribosomes translating the C-terminal region of secM can disrupt an RNA repressor helix that normally blocks secA translation initiation, derepressing the expression of secA. Translational pausing of secM at Pro-166 under secretion-limiting conditions increases the duration of the disruption and thus increases secA expression. This is controlled by interaction of the secM signal peptide with secA and the translocon, possibly by secA pulling the paused secM out of the ribosome. The arrest sequence (150-FXXXXWIXXXXGIRAGP-166) is sufficient to cause arrest of unrelated proteins. Elongation arrest can be alleviated by mutations in the 23S rRNA or in ribosomal protein L22.

Gene References into Functions

SecM can regulate its own synthesis by the force generated during folding, tuning ribosome activity to structure acquisition by a nascent polypeptide PMID: 25908824
Residues at positions between 161 and 166 of SecM showed decreased ribosome stalling. PMID: 21971705
RNA SsrA tagging of secretory monitor SecM would rescue ribosomes to avoid excessive jamming of the translation apparatus on stop-less secM mRNA PMID: 15494397
results suggest that cotranslational targeting of nascent SecM to the translocon plays previously unrecognized roles of facilitating the formation of functional SecA molecules PMID: 15713839
compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled ribosome complex PMID: 16713583
secM-encoded Pro166-tRNA functions as a nonpolypeptide element in fulfilling SecM's role as a secretion monitor. PMID: 16713584

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Subcellular Location

Cytoplasm, cytosol. Periplasm. Note=The active form is cytosolic, while the periplasmic form is rapidly degraded, mainly by the tail-specific protease.

Protein Families

SecM family

Database Links

KEGG: ecj:JW5007

STRING: 316385.ECDH10B_0079

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