Product Details

Purity

>85% (SDS-PAGE)

Target Names

AADAC

Uniprot No.

P22760

Alternative Names

AAAD_HUMAN; Aada; Aadac; Arylacetamide deacetylase (esterase); Arylacetamide deacetylase; CES5A1; DAC

Species

Homo sapiens (Human)

Source

Yeast

Protein Length

partial

Tag Info

N-terminal His-tagged
Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression vector recombined with the recombinant DNA was transfected into the yeast cells for expression. The recombinant DNA resulted from the fusion of the gene coding for the 24-399aa of the human AADAC protein and the N-terminal His tag gene. The product was purified and isolated to get the recombinant human AADAC protein with N-terminal His tag. This recombinant AADAC protein's purity reaches up to 85% determined by SAS-PAGE.

AADAC is a gene encoding a protein named arylacetamide deacetylase(abbreviated AADAC) and belongs to 'GDXG' lipolytic enzyme family. AADAC is a single serine esterase mainly expressed in liver, small intestine, colon, adrenal gland and bladder and to a lesser extent in the pancreas and adrenal gland. This enzyme is principally involved in the hydrolysis of several drugs containing the acetyl group. A study has demonstrated that AADAC is the principal enzyme involved in phenacetin hydrolysis with respect to CES enzyme contribution. AADAC may hydrolyze N-acetylarylamine drugs producing arylamine, which could cause toxic effects. Similar to the CES enzymes, AADAC also may be relevant in the preclinical development of inhaled drugs.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

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Applications : Enxymatic activity in vitro

Review: The hydrolysis activity of vicagrel to form 2-oxo-clopidogrel was measured in all recombinant human esterases, and data for these activities followed the Michaelis–Menten equations. Kinetic analyses of vicagrel hydrolase activity by recombinant CES1, CES2, and AADAC.

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Target Background

Function

Displays cellular triglyceride lipase activity in liver, increases the levels of intracellular fatty acids derived from the hydrolysis of newly formed triglyceride stores and plays a role in very low-density lipoprotein assembly. Displays serine esterase activity in liver. Deacetylates a variety of arylacetamide substrates, including xenobiotic compounds and procarcinogens, converting them to the primary arylamide compounds and increasing their toxicity.

Gene References into Functions

that the AADAC gene c.361+1G>A variant may be a potential candidate factor for Tourette syndrome development PMID: 29253601
Data suggest that AADAC in hepatocyte microsomes hydrolyzes ketoconazole, a synthetic imidazole antifungal agent, to N-deacetyl ketoconazole and thus triggers hepatocellular toxicity. PMID: 27422753
AADAC deletion is a candidate susceptibility factor for Gilles de la Tourette Syndrome PMID: 26444075
Translational N-glycosylation of AADAC plays a crucial role in regulating AADAC enzyme activity. PMID: 24125761
Lipolysis of cellular TG and VLDL production were impaired in HCV infected cells during the early peak of viral infection. This was partially explained by an apparent deficiency of a putative TG lipase, arylacetamide deacetylase (AADAC). PMID: 23542347
An AADAC*3 allele yields decreased enzyme activity in liver microsomes. PMID: 22415931
human AADAC was the enzyme responsible for the deacetylation of rifamycins and would affect the induction rate of drug-metabolizing enzymes by rifamycins and their induced hepatotoxicity. PMID: 21856291
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
two intrinsic endoplasmic reticulum (ER) proteins, 11beta-hydroxysteroid dehydrogenase, isozyme 1 (11beta-HSD) and the 50-kDa esterase (E3), sharing some amino acid sequence motifs in their N-terminal transmembrane (TM) domains. PMID: 10318829