DAD1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P61803 |
---|---|
Clone Names | 100712271 |
Gene ID | 1603 |
---|---|
Other Names | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1, Oligosaccharyl transferase subunit DAD1, Defender against cell death 1, DAD-1, DAD1 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | DAD1 (HGNC:2664) |
---|---|
Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853, PubMed:31831667). N- glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B- containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853). |
Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
DAD1, the defender against apoptotic cell death, wasinitially identified as a negative regulator of programmed celldeath in the temperature sensitive tsBN7 cell line. The DAD1protein disappeared in temperature-sensitive cells following ashift to the nonpermissive temperature, suggesting that loss of theDAD1 protein triggered apoptosis. DAD1 is believed to be a tightlyassociated subunit of oligosaccharyltransferase both in the intactmembrane and in the purified enzyme, thus reflecting the essentialnature of N-linked glycosylation in eukaryotes. [provided byRefSeq].
References
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :Kulke, M.H., et al. Genes Chromosomes Cancer 47(7):591-603(2008)Shibatani, T., et al. Biochemistry 44(16):5982-5992(2005)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.