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caveolin-1 siRNA and shRNA Plasmids (h2)

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Datasheets
  • Target species: human; for corresponding mouse product, see caveolin-1 siRNA and shRNA Plasmids (m)
  • Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
  • For independent verification of caveolin-1 gene silencing results, individual duplex components or plasmids are also available upon request
  • Suitable control antibody: caveolin-1 Antibody (7C8): sc-53564 is recommended as control antibody for monitoring of caveolin-1 expression knockdown by Western blotting or immunofluorescence
  • Get details about si/shRNA Gene Silencers
  • Gene silencers are intended for noncommercial and research use only. Click here for details.

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Description

  • 10 µM, 50-100 transfections
  • siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
  • for independent verification of caveolin-1 gene silencing results, individual siRNA duplex components are also available upon request
  • suitable control antibody: caveolin-1 Antibody (7C8): sc-53564
  • sqRT-PCR Primer also available: caveolin-1 (h2)-PR: sc-44202-PR, 20 µl
  • Get details about si/shRNA Gene Silencers

See Protocol Online.

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Description

  • 20 µl of each primer set is provided at a concentration of 10 µM
  • caveolin-1 Primer (h2) includes A set (forward & reverse) and B set (forward & reverse) primers
  • Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Description

  • 20 µg, up to 20 transfections
  • shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
  • shRNA sequences correspond to caveolin-1 siRNA Gene Silencer sequences
  • provided as transfection-ready purified plasmid DNA target-specific gene silencing
  • after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
  • shRNA Plasmids are intended only for non-commercial use.
  • suitable control antibody: caveolin-1 Antibody (7C8): sc-53564
  • sqRT-PCR Primer also available: caveolin-1 (h2)-PR: sc-44202-PR, 20 µl

See Protocol Online.

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Description

  • 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
  • Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
  • Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
  • shRNA sequences correspond to caveolin-1 siRNA Gene Silencer sequences
  • After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
  • Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
  • Lentiviral Particles are intended for non-commercial use.

See Protocol Online.

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Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

caveolin-1 siRNA (h2)

sc-44202
10 µM
$288.00

caveolin-1 (h2)-PR

sc-44202-PR
10 µM, 20 µl
$32.00

caveolin-1 shRNA Plasmid (h2)

sc-44202-SH
20 µg
$581.00

caveolin-1 shRNA (h2) Lentiviral Particles

sc-44202-V
200 µl
$698.00