Anti-EDG2 antibody [1G6]
| Name | Anti-EDG2 antibody [1G6] |
|---|---|
| Supplier | Abcam |
| Catalog | ab119245 |
| Prices | $384.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 1G6 |
| Applications | WB FC IHC-P |
| Species Reactivities | Human |
| Antigen | Recombinant full length Human EDG2 (NP_001392) produced in HEK293T cell |
| Description | Mouse Monoclonal |
| Gene | LPAR1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of EDG2 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119245, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![All lanes : Anti-EDG2 antibody [1G6] (ab119245) at 1/2000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with the pCMV6-ENTRY EDG2 cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11651_EDG2-Primary-antibodies-ab119245-3.jpg)
All lanes : Anti-EDG2 antibody [1G6] (ab119245) at 1/2000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with the pCMV6-ENTRY EDG2 cDNALysates/proteins at 5 µg per lane.
HEK293T cells transfected with either EDG2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained with 1/100 ab119245, and then analyzed by flow cytometry.