Anti-EEF2 antibody
| Name | Anti-EEF2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab33523 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Rat, Human, Zebrafish, Chicken, Hamster, Bovine, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human EEF2 |
| Description | Rabbit Polyclonal |
| Gene | EEF2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : Pancreas (Mouse) Tissue Lysate (ab29363)Lane 4 : Ovary (Mouse) Tissue Lysate - normal tissue Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 6 : Liver (Rat) Tissue Lysate - normal tissueLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
ICC/IF image of ab33523 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33523, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate (20ug)Lane 3 : Zebrafish heart homogenate (20ug)Lane 4 : Zebrafish liver homogenate (20ug)Lane 5 : Zebrafish skeletal muscle homogenate (20ug)Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (20ug)SecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of EEF2 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33523, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![EEF2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to EEF2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33523.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 95kDa; EEF2, non-specific band: 135kDa,due to background seen in No ab control lane (2).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/11864_EEF2-Primary-antibodies-ab33523-4.jpg)
EEF2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to EEF2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab33523.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 95kDa; EEF2, non-specific band: 135kDa,due to background seen in No ab control lane (2).
Product References
Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to - Homodimerization of RBPMS2 through a new RRM-interaction motif is necessary to
Sagnol S, Yang Y, Bessin Y, Allemand F, Hapkova I, Notarnicola C, Guichou JF, Faure S, Labesse G, de Santa Barbara P. Nucleic Acids Res. 2014 Sep;42(15):10173-84.
Investigation of adipocyte proteome during the differentiation of brown - Investigation of adipocyte proteome during the differentiation of brown
Kamal AH, Kim WK, Cho K, Park A, Min JK, Han BS, Park SG, Lee SC, Bae KH. J Proteomics. 2013 Dec 6;94:327-36.
Proteomic analysis of chikungunya virus infected microgial cells. - Proteomic analysis of chikungunya virus infected microgial cells.
Abere B, Wikan N, Ubol S, Auewarakul P, Paemanee A, Kittisenachai S, Roytrakul S, Smith DR. PLoS One. 2012;7(4):e34800.