EEF2

Gene Symbol EEF2
Entrez Gene 1938
Alt Symbol EEF-2, EF-2, EF2, SCA26
Species Human
Gene Type protein-coding
Description eukaryotic translation elongation factor 2
Other Description elongation factor 2|polypeptidyl-tRNA translocase
Swissprots D6W618 P13639 Q58J86 B2RMP5
Accessions EAW69274 EAW69275 P13639 AK297398 BAG59838 AK298498 BAG60705 AK299225 BAG61260 AU118535 AY942181 AAX34409 BC006547 AAH06547 BC024689 AAH24689 BC126259 AAI26260 BC136313 AAI36314 HQ258667 ADR83418 M19997 AAA50388 X51466 CAA35829 Z11692 CAA77750 NM_001961 NP_001952
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Subcellular Location Cytoplasm. Nucleus {ECO:0000269|PubMed:24648518}. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. {ECO:0000269|PubMed:24648518}.
Top Pathways Oxytocin signaling pathway, AMPK signaling pathway

Phospho-eEF2 (Thr56) Antibody - 2331 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6 cells, untreated or forskolin-treated (10 µM for 60 minutes), using Phospho-eEF2 (Thr56) Antibody (upper) or eEF2 Antibody #2332 (lower).

eEF2 Antibody - 2332 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6 cells, untreated or forskolin-treated (10 µM for 60 minutes), using Phospho-eEF2 (Thr56) Antibody (upper) or eEF2 Antibody #2332 (lower).

EF-2 (C-14) - sc-13004 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EF-2 (C-14): sc-13004. Western blot analysis of EF-2 expression in HeLa (A), HL-60 (B), NIH/3T3 (C), PC-12 (D) and BC3H1 (E) whole cell lysates and rat liver tissue extract (F).

EF-2 (C-9) - sc-166415 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EF-2 (C-9): sc-166415. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

EF-2 (F-9) - sc-166409 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EF-2 (F-9): sc-166409. Western blot analysis of EF-2 expression in NIH/3T3 whole cell lysate.

EF-2 (H-118) - sc-25634 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EF-2 (H-118): sc-25634. Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic and membrane staining of follicle cells and ovarian stroma cells.

EF-2 (P-19) - sc-13003 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EF-2 (P-19): sc-13003. Western blot analysis of EF-2 expression in HeLa (A), HL-60 (B), NIH/3T3 (C) and PC-12 (D) whole cell lysates and rat liver (E) extract.

EF-2 (H-8) - sc-390014 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EF-2 (H-8): sc-390014. Western blot analysis of EF-2 expression in PC-12 (A) and BC3H1 (B) whole cell lysates.

Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] - ab82981 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP833Y
All lanes : Anti-EEF2 (phospho T56 + T58) antibody [EP833Y] (ab82981) at 1/2000 dilutionLane 1 : Untreated 293 cell lysateLane 2 : Serum starved 293 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-EEF2 (phospho T56) antibody - ab53114 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2 (phospho T56) antibody (ab53114) at 1/300 dilutionLane 1 : extract from NIH 3T3 cells treated with serum (10%, 30mins)Lane 2 : extract from NIH 3T3 cells treated with serum (10%, 30mins) with immunising peptide

Anti-EEF2 (phospho T56) antibody - ab115165 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2 (phospho T56) antibody (ab115165) at 1 µg/mlLane 1 : K562 cell extractLane 2 : K562 cell extract treated with AnisomycinLane 3 : NIH3T3 cell extractLane 4 : NIH3T3 cell extract treated with Anisomycin

Anti-EEF2 antibody - ab33523 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2 antibody (ab33523) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-EEF2 antibody - ab40812 from Abcam

Prices $381.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-EEF2 antibody (ab40812) at 1/50000 dilution + 10ug HEK293

Anti-EEF2 antibody - ab33208 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2 antibody (ab33208) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 7 : SHSY-5Y (Human Neuroblastoma) Whole Cell LysateLane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-EEF2 antibody - ab53139 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EEF2 antibody (ab53139) at 1/300 dilutionLane 1 : NIH/3T3 cells treated with serum (10%, 30mins), no immunising peptideLane 2 : NIH/3T3 cells treated with serum (10%, 30mins), with immunising peptide

Anti-EEF2 antibody - ab84755 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon carcinoma, using 1/100 ab84755 in conjunction with 1/100 Goat anti-Rabbit IgG conjugated with a fluorescent dye (red).

Anti-EEF2 antibody [4B3-G7-H5] - ab131202 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4B3-G7-H5
Immunocytochemical staining of HeLa cells using anti-eEF2 antibody (ab131202) at a dilution of 1:200. Cells were fixed with ice cold methanol.

Anti-EEF2 antibody [5B6] - ab130187 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5B6
All lanes : Anti-EEF2 antibody [5B6] (ab130187) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : Hela cell lysateLane 3 : HEK293 cell lysateLane 4 : A431 cell lysate

Anti-EEF2 antibody [ELL2A11H2] - ab50748 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ELL2A11H2
Anti-EEF2 antibody [ELL2A11H2] (ab50748) + immunizing peptide

Anti-EEF2 antibody [EP723Y] - ab40762 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP723Y
Anti-EEF2 antibody [EP723Y] (ab40762) at 1/1000 dilution + HEK293 cell lysate at 10 µg

Anti-EEF2 antibody [EP880Y] - ab75748 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP880Y
EEF2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit monoclonal to EEF2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75748.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 95kDa; EEF2

Anti-EEF2 / Elongation Factor 2 Antibody (N-Terminus) - LS-C6487 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-EEF2 / Elongation Factor 2 Antibody (phospho-Thr56) - LS-C63114 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-EEF2 / Elongation Factor 2 Antibody (phospho-Thr56/Thr58, clone EP833Y) - LS-C105242 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP833Y
Western blot analysis on 293 cell lysates using anti-Phospho-eEF2 (pT56/58) antibody (LS-C105242), 1:2000 dilution. Cells were either (A) untreated (B) starved.

Anti-EEF2 / Elongation Factor 2 Antibody - LS-C285493 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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