Anti-epithelial Sodium Channel alpha antibody
| Name | Anti-epithelial Sodium Channel alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab65710 |
| Prices | $379.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P IHC-F ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide to an internal sequence (Human) conjugated to an immunogenic carrier protein |
| Blocking Peptide | epithelial Sodium Channel alpha peptide |
| Description | Rabbit Polyclonal |
| Gene | SCNN1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab65710 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65710, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.