Anti-Fas Ligand antibody
| Name | Anti-Fas Ligand antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68338 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic peptide corresponding to a sequence mapping at the C-terminus of human Fas Ligand, different to the related mouse sequence by three amino acids |
| Description | Rabbit Polyclonal |
| Gene | FASLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Fas Ligand antibody (ab68338) at 1 µg/ml + HeLa cell lysate
ab68338 staining Fas Ligan in human intestinal cancer tissue by Immunohistochemistry (formalin fixed, paraffin embedded section). Primary antibody used at 2µg/ml.
Anti-Fas Ligand antibody (ab68338) at 1/500 dilution + Mouse serum at 50 µgSecondaryHRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab68338 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68338 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Yeh CN, Chung WH, Su SC, Chen YY, Cheng CT, Lin YL, Chang WC, Chung-Yee Hui R, Chiang KC, Chen TW, Jan YY, Chen CW, Chen TJ, Yang CH, Hung SI. J Invest Dermatol. 2014 Nov;134(11):2768-75.
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Gonzalez-Rodriguez A, Reibert B, Amann T, Constien R, Rondinone CM, Valverde AM. Dis Model Mech. 2014 Sep;7(9):1093-100.
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