Anti-Fibronectin antibody

• Supplier: Abcam
• Catalog: ab23750
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA RIA ICC/IF ICC/IF IHC-F WB IHC-P
• Species Reactivities: Mouse, Human, Rat
• Antigen: Purified fibronectin from murine plasma
• Description: Rabbit Polyclonal
• Gene: FN1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab23750 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23750, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab23750 staining fibronectin in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary antibody.See Abreview

Anti-Fibronectin antibody (ab23750) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

ab23750 staining Fibronectin in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence. Cells were formaldehyde fixed, blocked in 2% BSA for 45 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, was used undiluted as secondary. The nuclei were stained with DAPI.

ab23750 (2ug/ml) staining Fibronectin in human aorta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining Fibronectin in the cytoplasm and extracellularly in the connective tissue.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

ab23750 staining Fibronectin in Rat skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Immunoblock® (5 µg/ml) for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour 30 minutes at 25°C. An undiluted Goat anti-rabbit HRP/DAB polydetector polyclonal was used as the secondary antibody.See Abreview

Product References

Establishment of a human 3D lung cancer model based on a biological tissue matrix - Establishment of a human 3D lung cancer model based on a biological tissue matrix

Stratmann AT, Fecher D, Wangorsch G, Gottlich C, Walles T, Walles H, Dandekar T, Dandekar G, Nietzer SL. Mol Oncol. 2014 Mar;8(2):351-65.

Immunocompetent 3D model of human upper airway for disease modeling and in vitro - Immunocompetent 3D model of human upper airway for disease modeling and in vitro

Harrington H, Cato P, Salazar F, Wilkinson M, Knox A, Haycock JW, Rose F, Aylott JW, Ghaemmaghami AM. Mol Pharm. 2014 Jul 7;11(7):2082-91.

Induction of retinal ganglion-like cells from fibroblasts by adenoviral gene - Induction of retinal ganglion-like cells from fibroblasts by adenoviral gene

Meng F, Wang X, Gu P, Wang Z, Guo W. Neuroscience. 2013 Oct 10;250:381-93.

Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model - Molecular MRI of atherosclerotic plaque progression in an ApoE(-/-) mouse model

Wu X, Balu N, Li W, Chen Y, Shi X, Kummitha CM, Yu X, Yuan C, Lu ZR. Am J Nucl Med Mol Imaging. 2013 Sep 19;3(5):446-55. eCollection 2013.

Withania somnifera root extract inhibits mammary cancer metastasis and epithelial - Withania somnifera root extract inhibits mammary cancer metastasis and epithelial

Yang Z, Garcia A, Xu S, Powell DR, Vertino PM, Singh S, Marcus AI. PLoS One. 2013 Sep 12;8(9):e75069.

Geometry-driven cell organization determines tissue growths in scaffold pores: - Geometry-driven cell organization determines tissue growths in scaffold pores:

Joly P, Duda GN, Schone M, Welzel PB, Freudenberg U, Werner C, Petersen A. PLoS One. 2013 Sep 5;8(9):e73545.

IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from - IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from

Vijayan A, Guha D, Ameer F, Kaziri I, Mooney CC, Bennett L, Sureshbabu A, Tonner E, Beattie J, Allan GJ, Edwards J, Flint DJ. Int J Biochem Cell Biol. 2013 Dec;45(12):2774-85. doi:

PAI-1-regulated miR-21 defines a novel age-associated fibrogenic pathway in - PAI-1-regulated miR-21 defines a novel age-associated fibrogenic pathway in

Ardite E, Perdiguero E, Vidal B, Gutarra S, Serrano AL, Munoz-Canoves P. J Cell Biol. 2012 Jan 9;196(1):163-75.

Effects of creatine and exercise on skeletal muscle of FRG1-transgenic mice. - Effects of creatine and exercise on skeletal muscle of FRG1-transgenic mice.

Ogborn DI, Smith KJ, Crane JD, Safdar A, Hettinga BP, Tupler R, Tarnopolsky MA. Can J Neurol Sci. 2012 Mar;39(2):225-31.

Role of epithelial integrin-linked kinase in promoting intestinal inflammation: - Role of epithelial integrin-linked kinase in promoting intestinal inflammation:

Assi K, Patterson S, Dedhar S, Owen D, Levings M, Salh B. BMC Immunol. 2011 Aug 1;12:42.