Anti-Fibronectin antibody [2F12]
| Name | Anti-Fibronectin antibody [2F12] |
|---|---|
| Supplier | Abcam |
| Catalog | ab25583 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2F12 |
| Applications | ELISA ICC/IF ICC/IF WB |
| Species Reactivities | Human |
| Antigen | The details of the immunogen for this antibody are not available |
| Description | Mouse Monoclonal |
| Gene | FN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-Fibronectin antibody [2F12] (ab25583) at 1/2000 dilution + TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_2/19225_Fibronectin-Primary-antibodies-ab25583-2.jpg)
Anti-Fibronectin antibody [2F12] (ab25583) at 1/2000 dilution + TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ICC/IF image of ab25583 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25583 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.