Anti-FMRP antibody

• Supplier: Abcam
• Catalog: ab27455
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB IHC-F IHC-P
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 600 to the C-terminus of Human FMRP
• Description: Rabbit Polyclonal
• Gene: FMR1
• Conjugate: Unconjugated

Product images

All lanes : Anti-FMRP antibody (ab27455) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

IHC image of FMRP staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27455, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ab27455 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27455 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

A functional requirement for PAK1 binding to the KH(2) domain of the fragile X - A functional requirement for PAK1 binding to the KH(2) domain of the fragile X

Say E, Tay HG, Zhao ZS, Baskaran Y, Li R, Lim L, Manser E. Mol Cell. 2010 Apr 23;38(2):236-49.

Fragile X protein family member FXR1P is regulated by microRNAs. - Fragile X protein family member FXR1P is regulated by microRNAs.

Cheever A, Blackwell E, Ceman S. RNA. 2010 Aug;16(8):1530-9.

Discrimination of common and unique RNA-binding activities among Fragile X mental - Discrimination of common and unique RNA-binding activities among Fragile X mental

Darnell JC, Fraser CE, Mostovetsky O, Darnell RB. Hum Mol Genet. 2009 Sep 1;18(17):3164-77.