FMR1

Gene Symbol FMR1
Entrez Gene 2332
Alt Symbol FMRP, FRAXA, POF, POF1
Species Human
Gene Type protein-coding
Description fragile X mental retardation 1
Other Description fragile X mental retardation protein 1
Swissprots G8JL90 D3DWT0 D3DWT2 Q5PQZ6 A6NNH4 Q16578 Q99054 Q06787 D3DWT1
Accessions AAA62452 AAA62453 AAA62454 AAA62455 AAA62456 AAA62457 AAA62458 AAA62459 AAA62460 AAA62461 AAA62462 AAA62463 AAA62464 AAA62465 AAA62466 AAA62467 AAA62468 AAA62469 AAB18828 AAB18829 AAB18830 AAB18831 AAB18832 AAB18833 AAD14228 EAW61293 EAW61294 EAW61295 EAW61296 EAW61297 EAW61298 EAW61299 EAW61300 EAW61301 EAW61302 EAW61303 Q06787 AB209188 BAD92425 AK294104 BAG57439 AK302707 BAG63929 BC038998 AAH38998 BC086957 AAH86957 BQ213316 BQ287827 BQ417290 BX498094 CK825936 CN315330 CX871385 KC774027 AGO02164 KC774028 AGO02165 KC774029 AGO02166 KF856234 AHI43787 KJ534836 AHW56476 KJ534837 AHW56477 KJ534838 AHW56478 M67468 AAA52458 S65791 AAB28395 X69962 CAA49586 NM_001185075 NP_001172004 NM_001185076 NP_001172005 NM_001185081 NP_001172010 NM_001185082 NP_001172011 NM_002024 NP_002015 NR_033699 NR_033700
Function Translation repressor. Component of the CYFIP1-EIF4E- FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA- binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C). {ECO:0000250}.
Subcellular Location Cytoplasm. Nucleus, nucleolus.
Tissue Specificity Highest levels found in neurons, brain, testis, placenta and lymphocytes. Also expressed in epithelial tissues and at very low levels in glial cells. {ECO:0000269|PubMed:8401578, ECO:0000269|PubMed:8504300}.
Top Pathways RNA transport

FMRP (D14F4) Rabbit mAb - 7104 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D14F4
Western blot analysis of extracts from various cell lines using FMRP (D14F4) Rabbit mAb.

FMRP (G468) Antibody - 4524 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using FMRP (G468) Antibody.

FMRP Antibody - 4317 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using FMRP Antibody.

FMR1 (H-120) - sc-28739 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FMR1 (H-120): sc-28739. Western blot analysis of FMR1 expression in 293T whole cell lysate.

FMR1 (K-16) - sc-10547 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FMR1 (K-16): sc-10547. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells, subset of cells in granular layer and subset of cells in molecular layer.

FMR1 (G-16) - sc-10548 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

FMR1 (148.1) - sc-101048 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
FMR1 (148.1): sc-101048. Western blot analysis of FMR1 expression in 293T (A) and Jurkat (B) whole cell lysates.

Anti-FMRP [EPR12643] antibody - ab191411 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12643
All lanes : Anti-FMRP [EPR12643] antibody (ab191411) at 1/10000 dilutionLane 1 : 293 cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-FMRP (phospho S499) antibody - ab48127 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FMRP (phospho S499) antibody (ab48127) at 2 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Mouse Brain Tissue LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FMRP antibody - ab17722 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-MCPG sodium salt (ab120252), by ICC/IF. Decrease of FMRP expression correlates with increased concentration of (R,S)-MCPG sodium salt, as described in literature.The cells were incubated at 37°C for 2h in media containing different concentrations of ab120252 ((R,S)-MCPG sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-FMRP antibody - ab27455 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FMRP antibody (ab27455) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate SecondaryGoat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

Anti-FMRP antibody - ab82144 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-FMRP antibody (ab82144) at 0.1 µg/ml + KELLY cell lysate at 35 µgdeveloped using the ECL technique

Anti-FMRP antibody - ab91553 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-FMRP antibody (ab91553) at 1/100 dilution + Mouse lung tissue lysates at 35 µg

Anti-FMRP antibody [4G9] - ab130165 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4G9
Overlay histogram showing SH-SY5Y cells stained with ab130165 (red line). The cells were fixed with 4% paraformaldehyde (10 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130165, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-FMRP antibody [5B9] - ab128095 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5B9
All lanes : Anti-FMRP antibody [5B9] (ab128095) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY FMRP cDNALysates/proteins at 5 µg per lane.

Anti-FMRP antibody [EPR12643] (Alexa Fluor® 488) - ab199833 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12643
ab199833 staining FMRP in PC12 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199833 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FMRP antibody [EPR12643] (Alexa Fluor® 594) - ab202230 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12643
ab202230 staining FMRP in PC12 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202230 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FMRP antibody [EPR12643] (Alexa Fluor® 647) - ab199834 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12643
ab199834 staining FMRP in PC12 cells. The cells were fixed with 4% formaldehyde (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199834 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-FMRP antibody - Carboxyterminal end - ab69815 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-FMRP antibody - Carboxyterminal end (ab69815) at 1/1000 dilution + Mouse brain tissue lysate - whole at 30 µgSecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FMR1 / FMRP Antibody (N-Terminus, clone 1C3) - LS-C15265 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1C3

Anti-FMR1 / FMRP Antibody - LS-C121832 from LifeSpan Bioscience

Prices $575.00
Sizes 10 assy
Host Mouse
Clonality Monoclonal

Anti-FMR1 / FMRP Antibody - LS-C129158 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-FMR1 / FMRP Antibody (N-Terminus, clone 6A15) - LS-C82231 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6A15
Immunoprecipitation - Drosophila FMR1 antibody [6A15] using tissue lysate from Drosophila cellularizing embryos

Anti-FMR1 / FMRP Antibody (aa121-221, clone 10C270, Azide-free) - LS-C183631 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10C270

Anti-FMR1 / FMRP Antibody (aa421-470) - LS-C135098 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
FMR1 antibody LS-C135098 Western blot of Rat Lung lysate.
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