Anti-FOXP3 antibody
| Name | Anti-FOXP3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10563 |
| Prices | $391.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC WB IHC-F ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Human, Monkey |
| Antigen | Synthetic peptide corresponding to Human FOXP3 aa 400 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Human FOXP3 peptide |
| Description | Rabbit Polyclonal |
| Gene | FOXP3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescent imaging of human cells (U2OS) with ab10563 (5 ug/ml) confirms the specificity of this anti-FOXP3 antibody, which recognises nuclear signal only; with no background cytoplasmic or nucleolar staining. IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour). Primary antibody used at 5ug/ml in 5% milk / 0.2% TWEEN for 1 hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.
FoxP3 immunostaining on a frozen section from human tonsil. Original magnification x100. Nuclear staining was observed.
Lanes 1 - 6 : Anti-FOXP3 antibody (ab10563) at 2 µg/mlLane 7 : Lane 1 : 20µg HEK 293 lysateLane 2 : 20µg HEK 293 lysate over expressing human FOXP3Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab14151) at 1 µg/mlLane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab14151) at 1 µg/mlLane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab14151) at 1 µg/mlLane 7 : 20µg HEK 293 lysateSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/mlPerformed under reducing conditions.
Product References
FOXP3 over-expression inhibits melanoma tumorigenesis via effects on - FOXP3 over-expression inhibits melanoma tumorigenesis via effects on
Tan B, Anaka M, Deb S, Freyer C, Ebert LM, Chueh AC, Al-Obaidi S, Behren A, Jayachandran A, Cebon J, Chen W, Mariadason JM. Oncotarget. 2014 Jan 15;5(1):264-76.
Ex vivo expanded human regulatory T cells delay islet allograft rejection via - Ex vivo expanded human regulatory T cells delay islet allograft rejection via
Xiao F, Ma L, Zhao M, Huang G, Mirenda V, Dorling A, Lechler R, Lombardi G. PLoS One. 2014 Mar 3;9(3):e90387.
FOXP3+ lymphocyte density in pancreatic cancer correlates with lymph node - FOXP3+ lymphocyte density in pancreatic cancer correlates with lymph node
Jiang Y, Du Z, Yang F, Di Y, Li J, Zhou Z, Pillarisetty VG, Fu D. PLoS One. 2014 Sep 5;9(9):e106741.
Immunosuppression affects CD4+ mRNA expression and induces Th2 dominance in the - Immunosuppression affects CD4+ mRNA expression and induces Th2 dominance in the
Kosmidis M, Dziunycz P, Suarez-Farinas M, Muhleisen B, Scharer L, Lauchli S, Hafner J, French LE, Schmidt-Weber C, Carucci JA, Hofbauer GF. J Immunother. 2010 Jun;33(5):538-46.
Interleukin-1 as a phenotypic immunomodulator in keratinizing squamous metaplasia - Interleukin-1 as a phenotypic immunomodulator in keratinizing squamous metaplasia
Chen YT, Nikulina K, Lazarev S, Bahrami AF, Noble LB, Gallup M, McNamara NA. Am J Pathol. 2010 Sep;177(3):1333-43.
Aire and Foxp3 expression in a particular microenvironment for T cell - Aire and Foxp3 expression in a particular microenvironment for T cell
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Phosphorus overload and PTH induce aortic expression of Runx2 in experimental - Phosphorus overload and PTH induce aortic expression of Runx2 in experimental
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Progression of cutaneous squamous cell carcinoma in immunosuppressed patients is - Progression of cutaneous squamous cell carcinoma in immunosuppressed patients is
Muhleisen B, Petrov I, Gachter T, Kurrer M, Scharer L, Dummer R, French LE, Hofbauer GF. Histopathology. 2009 Jul;55(1):67-76.
Increased T-regulatory cells within lymphocyte follicles in moderate COPD. - Increased T-regulatory cells within lymphocyte follicles in moderate COPD.
Plumb J, Smyth LJ, Adams HR, Vestbo J, Bentley A, Singh SD. Eur Respir J. 2009 Jul;34(1):89-94.
FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of - FOXP3+ Tregs and B7-H1+/PD-1+ T lymphocytes co-infiltrate the tumor tissues of
Ghebeh H, Barhoush E, Tulbah A, Elkum N, Al-Tweigeri T, Dermime S. BMC Cancer. 2008 Feb 23;8:57.