FOXP3

Gene Symbol FOXP3
Entrez Gene 50943
Alt Symbol AIID, DIETER, IPEX, JM2, PIDX, XPID
Species Human
Gene Type protein-coding
Description forkhead box P3
Other Description FOXP3delta7|forkhead box protein P3|immune dysregulation, polyendocrinopathy, enteropathy, X-linked|immunodeficiency, polyendocrinopathy, enteropathy, X-linked|scurfin
Swissprots Q14DD8 O60827 Q9BZS1 Q4ZH51 B9UN80 B7ZLG0 A5HJT1
Accessions CAY55968 CCF77062 CDH61704 EAW50671 EAW50672 EAW50673 Q9BZS1 AF277993 AAG53607 AJ005891 CAA06748 AK292052 BAF84741 AK299988 BAG61809 BC111853 AAI11854 BC113401 AAI13402 BC113403 AAI13404 BC143785 AAI43786 BC143786 AAI43787 BQ184335 DB342786 DQ010327 AAY27088 EF534714 ABQ15210 EU855812 ACJ46653 HQ258232 ADR82986 XM_006724533 XP_006724596 XM_011543915 XP_011542217 XM_011543916 XP_011542218 XM_011543917 XP_011542219 XM_011543918 XP_011542220 XM_011543919 XP_011542221 NM_001114377 NP_001107849 NM_014009 NP_054728
Function Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon- gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone ace
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00089, ECO:0000269|PubMed:17360565, ECO:0000269|PubMed:18354202, ECO:0000269|PubMed:22678915, ECO:0000269|PubMed:23396208, ECO:0000269|PubMed:23973222, ECO:0000269|PubMed:23973223}. Cytoplasm {ECO:0000269|PubMed:22678915}. Note=Predominantly expressed in the cytoplasm in activated conventional T-cells whereas predominantly expressed in the nucleus in regulatory T-cells (Treg). The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells (By similarity). {ECO:0000250|UniProtKB:Q99JB6, ECO:0000269|PubMed:22678915}.
Top Pathways Inflammatory bowel disease (IBD)

FoxP3 (D6O8R) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 13282 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6O8R
Flow cytometric analysis of murine spleen lymphocytes using FoxP3 (D6O8R) Rabbit mAb (Alexa Fluor ® 488 Conjugate) #13282 versus a CD25 antibody. Analysis was performed on gated a CD4+ T cell population.

FoxP3 (D6O8R) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 13399 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6O8R
Flow cytometric analysis of murine spleen lymphocytes using FoxP3 (D6O8R) Rabbit mAb (Alexa Fluor ® 647 Conjugate) #13399 versus a CD25 antibody. Analysis was performed on a gated CD4+ T cell population.

FoxP3 (D6O8C) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 12853 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6O8C
Flow cytometric analysis of human peripheral blood mononuclear cells using FoxP3 (D6O8C) Rabbit mAb (Alexa Fluor ® 488 Conjugate) versus an anti-human CD25 antibody, gated on either CD4+ T cells (right) or CD4- T cells (left).

FoxP3 (D6O8C) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 12729 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6O8C
Flow cytometric analysis of human peripheral blood mononuclear cells using FoxP3 (D6O8C) Rabbit mAb (Alexa Fluor ® 647 Conjugate) versus an anti-human CD25 antibody, gated on either CD4+ T cells (left) or CD4- T cells (right).

FoxP3 (D6O8R) Rabbit mAb - 12653 from Cell Signaling Technology

Prices $109.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D6O8R
Immunohistochemical analysis of paraffin-embedded mouse thymus using FoxP3 (D6O8R) Rabbit mAb.

FoxP3 (D25D4) Rabbit mAb - 5298 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D25D4
Western blot analysis of extracts from COS-7 cells, mock transfected or transfected with human FoxP3, and human thymus, using FoxP3 (D25D4) Rabbit mAb.

FoxP3 (D6O8C) Rabbit mAb - 12632 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D6O8C
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with human FoxP3 (hFoxP3; +), and human thymus using FoxP3 (D8O6C) Rabbit mAb.

FOXP3 (150D/E4) - sc-130666 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FOXP3 (150D/E4) PE: sc-130666 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG<sub>1</sub>: sc-2866.

FOXP3 (2A11G9) - sc-53876 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FOXP3 (2A11G9): sc-53876. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.

FOXP3 (3G3) - sc-52899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

FOXP3 (6H3C5H3) - sc-65988 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FOXP3 (6H3C5H3): sc-65988. Western blot analysis of human recombinant FOXP3 fusion protein.

FOXP3 (F-9) - sc-166212 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
FOXP3 (F-9): sc-166212. Western blot analysis of FOXP3 expression in Jurkat whole cell lysate.

FOXP3 (H-190) - sc-28705 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
FOXP3 (H-190): sc-28705. Western blot analysis of FOXP3 expression in RAW 264.7 nuclear extract.

FOXP3 (K-13) - sc-31739 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

FOXP3 (N-12) - sc-21072 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FOXP3 (N-12): sc-21072. Western blot analysis of FOXP3 expression in human brain tissue extract.

FOXP3 (V-17) - sc-31738 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
FOXP3 (V-17): sc-31738. Western blot analysis of FOXP3 expression in rat brain tissue extract.

Anti-FOXP3 [EPR15038-69] antibody - C-terminal - ab191416 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR15038-69
Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling FOXP3 with ab191416 at 1/250 dilution (0.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.Inset: Negative control: using PBS instead of primary antibody.

Anti-FOXP3 (exon 2 deleted) antibody [16J4G6] - ab72008 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 16J4G6
All lanes : Anti-FOXP3 (exon 2 deleted) antibody [16J4G6] (ab72008) at 1 µg/mlLane 1 : FOXP3 (exon 2 deleted) transfected 293 cell lysate Lane 2 : FOXP3 (wild type) transfected 293 cell lysate Observed band size : 40 kDa (why is the actual band size different from the predicted?)Additional bands at : 97 kDa. We are unsure as to the identity of these extra bands.

Anti-FOXP3 antibody - ab10563 from Abcam

Prices $391.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent imaging of human cells (U2OS) with ab10563 (5 ug/ml) confirms the specificity of this anti-FOXP3 antibody, which recognises nuclear signal only; with no background cytoplasmic or nucleolar staining.   IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour).  Primary antibody used at 5ug/ml in 5% milk / 0.2% TWEEN for 1 hour, secondary antibody for 30 minutes.  All blocking and incubation steps carried out at 37 degrees C. Nuclei were stained with Hoechst stain.

Anti-FOXP3 antibody - ab4728 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-FOXP3 antibody - ab128800 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Overlay histogram showing Jurkat cells stained with ab128800 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab128800, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (polyclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-FOXP3 antibody - ab10901 from Abcam

Prices $379.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXP3 antibody (ab10901) at 0.4 µg/mlLane 1 : 20µg HEK 293 lysateLane 2 : 20µg HEK 293 lysate over expressing human FOXP3Lane 3 : 20µg HEK 293 lysate over expressing mouse FOXP3Lane 4 : 20µg HEK 293 lysate with Human FOXP3 peptide (ab16809) at 1 µg/mlLane 5 : 20µg HEK 293 lysate over expressing human FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/mlLane 6 : 20µg HEK 293 lysate over expressing mouse FOXP3 with Human FOXP3 peptide (ab16809) at 1 µg/mlSecondaryLanes 4 - 6 : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 0.2 µg/mlPerformed under reducing conditions.

Anti-FOXP3 antibody - ab150743 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXP3 antibody (ab150743) at 1 µg/mlLane 1 : HEK293 Whole Cell Lysate Overexpressing FOXP3 at 0.1 µgLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-FOXP3 antibody - ab86006 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXP3 antibody (ab86006) at 1/1000 dilution (in 5% non-fat milk-PBST solution incubated for 60 minutes)Lane 1 : uninduced culture of E. coli (plus anti thio at 1/5000 dilution) Lane 2 : induced culture of E. coli (plus anti thio at 1/5000 dilution) Secondaryanti-Rabbit IgG HRP conjugate incubated for 60 minutes at 1/1000 dilution

Anti-FOXP3 antibody - ab54501 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-FOXP3 antibody (ab54501) at 1/2000 dilutionLane 1 : Jurkat Tag cells transfected with the expression vectorLane 2 : Jurkat Tag cells transfected with the FOXP3 expression construct
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