Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with human FoxP3 (hFoxP3; +), and human thymus using FoxP3 (D8O6C) Rabbit mAb.
Flow cytometric analysis of human peripheral blood mononuclear cells gated on CD4+ lymphocytes, showing FoxP3 expression in CD25+ cells using FoxP3 (D6O8C) Rabbit mAb (left), and corresponding absence of signal in CD25+ cells using concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, (right). Anti-rabbit IgG (H+L), (F(ab') 2 ) Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody. Refer to the product specific protocol link indicated on the datasheet.