Anti-GAPDH antibody
| Name | Anti-GAPDH antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab37168 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ELISA ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human, Monkey |
| Antigen | Synthetic peptide, corresponding to 16 N terminal amino acids of Human GAPDH |
| Blocking Peptide | Human GAPDH peptide |
| Description | Rabbit Polyclonal |
| Gene | GAPDH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-GAPDH antibody (ab37168) at 0.125 µg/mlLane 2 : Anti-GAPDH antibody (ab37168) at 0.25 µg/mlLane 3 : Anti-GAPDH antibody (ab37168) at 0.5 µg/mlLane 1 : HeLa cell lysate with GAPDH peptideLane 2 : HeLa cell lysate with GAPDH peptideLane 3 : HeLa cell lysate with GAPDH peptideLysates/proteins at 15 µg per lane.Secondaryanti-rabbit IgG HRP at 1/10000 dilution
ICC/IF image of ab37168 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1mg/ml) overnight at +4øC. The secondary antibody (green) was anti-rabbit Alexa Fluor® 488 (ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.
ICC/IF image of ab37168 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37168, 1µg/ml) overnight at +4°C. The secondary antibody (green) was anti-Rabbit DyLight® 488 pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of GAPDH in HeLa cells using ab37168 at 10 ug/ml.
ab37168 at 10µg/ml staining GAPDH in human liver tissue by IHC
Product References
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Hou A, Voorhoeve PM, Lan W, Tin M, Tong L. Exp Cell Res. 2013 Nov 1;319(18):2781-9.
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