GAPDH

Gene Symbol GAPDH
Entrez Gene 2597
Alt Symbol G3PD, GAPD, HEL-S-162eP
Species Human
Gene Type protein-coding
Description glyceraldehyde-3-phosphate dehydrogenase
Other Description aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
Swissprots E7EUT4 Q53X65 P00354 P04406
Accessions AAA53191 AAP88932 AEQ28035 AEQ28036 AEQ28037 EAW88786 EAW88787 P04406 AB062273 BAB93466 AF261085 AAF99678 AJ844644 CAH59755 AJ844645 CAH59756 AK026525 AK299448 BAG61421 AK308198 AY007133 AAG01996 AY633612 AAV33305 BC001601 AAH01601 BC004109 AAH04109 BC009081 AAH09081 BC013310 AAH13310 BC020308 BC023632 AAH23632 BC025925 AAH25925 BC026907 AAH26907 BC029340 BC029618 AAH29618 BC083511 AAH83511 BE893087 BG724119 BI463134 BM763361 BT006893 AAP35539 BU155402 CR407671 CAG28599 DQ403057 ABD77190 DQ891557 ABM82483 DQ894744 ABM85670 EF036498 ABO65084 EU668321 ACF94474 HQ727992 AET97565 HY000136 HY004110 HY022295 HY046784 J02642 AAA52496 M17851 AAA86283 M28283 AAA52519 M33197 AAA52518 M36164 X01677 CAA25833 X53778 CAA37794 NM_001256799 NP_001243728 NM_001289745 NP_001276674 NM_001289746 NP_001276675 NM_002046 NP_002037
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibit
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:12829261}. Nucleus {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:12829261}. Membrane {ECO:0000269|PubMed:12829261}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. {ECO:0000250}.
Top Pathways Glycolysis / Gluconeogenesis, Alzheimer's disease, Biosynthesis of amino acids, Carbon metabolism

GAPDH (D16H11) XP ® Rabbit mAb (HRP Conjugate) - 8884 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16H11
Western blot analysis of extracts from NIH/3T3 and HeLa cells using GAPDH (D16H11) XP ® Rabbit mAb (HRP Conjugate).

GAPDH (14C10) Rabbit mAb (HRP Conjugate) - 3683 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 14C10
Western blot analysis of extracts from NIH/3T3, HeLa and C6 cells using GAPDH (14C10) Rabbit mAb (HRP Conjugate).

GAPDH (14C10) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 3906 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 14C10
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (14C10) (Alexa Fluor ® 488 Conjugate) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

GAPDH (14C10) Rabbit mAb (Biotinylated) - 5014 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 14C10
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using GAPDH (14C10) Rabbit mAb (Biotinylated).

GAPDH (14C10) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 3907 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone 14C10
Confocal immunofluorescent analysis of C2C12 cells using GAPDH (14C10) Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (fluorescent DNA dye).

GAPDH (14C10) Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 3964 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 14C10

GAPDH (D16H11) XP ® Rabbit mAb - 5174 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16H11
Western blot analysis of extracts from various cell lines using GAPDH (D16H11) XP ® Rabbit mAb.

GAPDH (14C10) Rabbit mAb - 2118 from Cell Signaling Technology

Prices $246.00, $637.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 14C10
Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb.

GAPDH (G-9) - sc-365062 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (G-9): sc-365062. Western blot analysis of GAPDH expression in Hep G2 whole cell lysate.

GAPDH (H-12) - sc-166574 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (H-12): sc-166574. Western blot analysis of GAPDH expression in non-transfected 293T: sc-117752 (A), human GAPDH transfected 293T: sc-113887 (B) and HeLa (C) whole cell lysates.

GAPDH (I-19) - sc-48166 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAPDH (I-19): sc-48166. Western blot analysis of GAPDH expression in non-transfected 293: sc-110760 (A), human GAPDH transfected 293: sc-110976 (B) and HeLa (C) whole cell lysates.

GAPDH (L-18) - sc-48167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAPDH (L-18): sc-48167. Western blot analysis of GAPDH expression in A549 (A), Hep G2 (B), KNRK (C), JAR (D), HeLa (E) and Raji (F) whole cell lysates.

GAPDH (L-20) - sc-31915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAPDH (L-20): sc-31915. Western blot analysis of GAPDH expression in A549 (A), Hep G2 (B), KNRK (C), JEG-3 (D), HeLa (E) and Raji (F) whole cell lysates.

GAPDH (N-14) - sc-20356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAPDH (N-14): sc-20356. Western blot analysis of GAPDH expression in A549 whole cell lysate.

GAPDH (V-18) - sc-20357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAPDH (V-18): sc-20357. Western blot analysis of GAPDH expression in non-transfected: sc-110760 (A) and human GAPDH transfected: sc-110976 (B) 293 whole cell lysates.

GAPDH (A-14) - sc-20358 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GAPDH (A-14): sc-20358. Western blot analysis of GAPDH expression in non-transfected: sc-117752 (A) and human GAPDH transfected: sc-113887 (B) 293T whole cell lysates.

GAPDH (A-3) - sc-137179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (A-3): sc-137179. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

GAPDH (D-6) - sc-166545 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (D-6): sc-166545. Western blot analysis of GAPDH expression in HeLa (A) and A549 (B) whole cell lysates.

GAPDH (FL-335) - sc-25778 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GAPDH (FL-335):sc-25778. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

GAPDH (0411) - sc-47724 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (0411): sc-47724. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

GAPDH (10B8) - sc-51905 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (10B8): sc-51905. Western blot analysis of GAPDH expression in HeLa whole cell lysate.

GAPDH (1D4) - sc-59540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 250 µl supernatant
Host Mouse
Clonality Monoclonal
GAPDH (1D4): sc-59540. Western blot analysis of GAPDH expression in Hep G2 (A), KNRK (B), JAR (C), HeLa (D) and Raji (E) whole cell lysates.

GAPDH (6C5) - sc-32233 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (6C5): sc-32233.Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

GAPDH (6F7) - sc-51907 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
GAPDH (6F7): sc-51907. Western blot analysis of GAPDH expression in HeLa whole cell lysate.

GAPDH (7B) - sc-69778 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
GAPDH (7B): sc-69778. Western blot analysis of GAPDH expression in non-transfected 293T: sc-117752 (A), human GAPDH transfected 293T: sc-113612 (B) and A549 (C) whole cell lysates.
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