GAPDH

Gene Symbol GAPDH
Entrez Gene 2597
Alt Symbol G3PD, GAPD, HEL-S-162eP
Species Human
Gene Type protein-coding
Description glyceraldehyde-3-phosphate dehydrogenase
Other Description aging-associated gene 9 protein|epididymis secretory sperm binding protein Li 162eP|peptidyl-cysteine S-nitrosylase GAPDH
Swissprots E7EUT4 Q53X65 P00354 P04406
Accessions AAA53191 AAP88932 AEQ28035 AEQ28036 AEQ28037 EAW88786 EAW88787 P04406 AB062273 BAB93466 AF261085 AAF99678 AJ844644 CAH59755 AJ844645 CAH59756 AK026525 AK299448 BAG61421 AK308198 AY007133 AAG01996 AY633612 AAV33305 BC001601 AAH01601 BC004109 AAH04109 BC009081 AAH09081 BC013310 AAH13310 BC020308 BC023632 AAH23632 BC025925 AAH25925 BC026907 AAH26907 BC029340 BC029618 AAH29618 BC083511 AAH83511 BE893087 BG724119 BI463134 BM763361 BT006893 AAP35539 BU155402 CR407671 CAG28599 DQ403057 ABD77190 DQ891557 ABM82483 DQ894744 ABM85670 EF036498 ABO65084 EU668321 ACF94474 HQ727992 AET97565 HY000136 HY004110 HY022295 HY046784 J02642 AAA52496 M17851 AAA86283 M28283 AAA52519 M33197 AAA52518 M36164 X01677 CAA25833 X53778 CAA37794 NM_001256799 NP_001243728 NM_001289745 NP_001276674 NM_001289746 NP_001276675 NM_002046 NP_002037
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibit
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:12829261}. Nucleus {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000269|PubMed:12829261}. Membrane {ECO:0000269|PubMed:12829261}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. {ECO:0000250}.
Top Pathways Glycolysis / Gluconeogenesis, Alzheimer's disease, Biosynthesis of amino acids, Carbon metabolism

PathScan ® Total GAPDH Sandwich ELISA Kit - 7157 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1. GAPDH protein is detected at varying levels from multiple cell lines using the PathScan® Total GAPDH Sandwich ELISA Kit #7157. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using GAPDH (D16H11) XP® Rabbit mAb #5174 are shown in the bottom figure.

SimpleChIP ® Human GAPDH Exon 1 Primers - 5516 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human GAPDH Exon 1 Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 60°C anneal/extension for 60 sec).

SimpleChIP ® Human GAPDH Promoter Primers - 4471 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human GAPDH Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

SimpleChIP ® Human GAPDH Intron 2 Primers - 4478 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human GAPDH Intron 2 Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

GAPDH PhosphoTracer ELISA Kit - ab119627 from Abcam

Category ELISA Kit
Sensitivity 25 pg/ml
Range 25 pg/ml - 6000 pg/ml
As shown, using the GAPDH assay kit, cellular GAPDH is readily detected in A431 cellular lysates, in either untreated cells (-), or cells treated with EGF (+).

GAPDH SimpleStep ELISA™ Kit - ab176642 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity < 5 ng/ml
Range 7 ng/ml - 700 ng/ml
Example of a typical GAPDH cell lysate dilution series.  Background-subtracted data values (mean +/- SD) are graphed.

Organelle Detection Western Blot Cocktail - ab133989 from Abcam

Category Cellular Assay
Prices $501.00
Sizes 200 µl
All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20. Lane 2-6 : Mouse heart homogenate Whole Tissue Lysate 10 µg Primary antibody: Lane 2 : Anti-Sodium Potassium ATPase antibody – Plasma Membrane Marker Lane 3 : Anti-ATP5A antibody – Mitochondrial Marker Lane 4 : Anti-GAPDH antibody – Cytosolic Marker Lane 5 : Anti-Histone H3 (di methyl K9) antibody – Nuclear Marker Lane 6 : Assembled Organelle Detection CocktailSecondary: ab131368 at 1/1000 dilution.

Human GAPDH ELISA Kit (Sandwich ELISA) - LS-F5733 from LifeSpan Bioscience

Category ELISA Kit
Prices $690.00
Sizes 1 plate
Sensitivity 1.56 ng/ml
Range 1.56-100 ng/ml

Mouse GAPDH ELISA Kit (Sandwich ELISA) - LS-F7579 from LifeSpan Bioscience

Category ELISA Kit
Prices $700.00
Sizes 1 plate
Sensitivity 0.156 ng/ml
Range 1.56-100 ng/ml

Mouse GAPDH ELISA Kit (Sandwich ELISA) - LS-F11502 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 0.312
Range 1.56-100 ng/ml

Human GAPDH ELISA Kit (Sandwich ELISA) - LS-F11503 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 0.15 ng/ml
Range 1.56-100 ng/ml

Goat anti-GAPDH Antibody, Affinity Purified - PLA0302 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-GAPDH Antibody, Affinity Purified - PLA0125 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunohistochemistry</B><BR/>Rabbit Anti-GAPDH Antibody, Affinity Purified: <B>Cat. No. PLA0125</B>: Detection of Human and Mouse GAPDH by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-GAPDH (<B>Cat. No. PLA0125</B>) used at a dilution of 1:200 (1 μg/mL). Detection: DAB

Human/Mouse/Rat Total GAPDH DuoSet IC ELISA - DYC5718-2 from R&D Systems

Category ELISA Kit
Prices $329.00, $649.00
Sizes 1 kit (for 2 Plates), 1 kit (for 5 Plates)
Range 156.00 - 10,000 pg/mL

Human GAPDH ELISA Kit - abx151626 from Abbexa

Category ELISA Kit
Sensitivity 0.57 ng/ml
Range 1.563 ng/ml - 100 ng/ml

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), Human, ELISA Kit - SEB932Hu from ARP American Research Products

Category ELISA Kit
Prices $685.00
Sizes 96 t
Sensitivity 0.57ng/mL
Range 1.56-100ng/mL

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Human, ELISA Kit - E01G0415 from ARP American Research Products

Category ELISA Kit
Prices $535.00, $735.00
Sizes 48 t, 96 t

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH/G3PDH), Human, ELISA Kit - CSB-E13911h from ARP American Research Products

Category ELISA Kit
Prices $550.00, $790.00, $791.00, $794.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t
Sensitivity 0.31 ng/ml
Range 1.25 ng/ml-80 ng/ml

Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Human, ELISA Kit - E1932h from ARP American Research Products

Category ELISA Kit
Prices $596.00
Sizes 96 t
Range 0.156-10 ng/mL

GAPDH (Human) Matched Antibody Pair - H00002597-AP51 from Acris Antibodies

Category Antibody Pair
Prices $610.00
Sizes 1 set

Custom Human GAPHD ELISA - ELH-GAPDH from RayBiotech

Category ELISA Kit
Prices $615.00, $1,107.00, $2,460.00
Sizes 1 plate kit, 2 plate kit, 5 plate kit

Human GAPDH ELISA Kit - HG0312 from NeoScientific

Category ELISA Kit
Prices $495.00
Sizes 96 t

Anserine GAPDH ELISA Kit - AG0312 from NeoScientific

Category ELISA Kit
Prices $495.00
Sizes 96 t

GAPDH, ELISA Kit - MBS2516079 from MyBioSource

Category ELISA Kit
Prices $410.00, $490.00, $2,040.00, $3,590.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
Sensitivity 0.938ng/mL
Range 1.563-100ng/mL
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