All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20. Lane 2-6 : Mouse heart homogenate Whole Tissue Lysate 10 µg Primary antibody: Lane 2 : Anti-Sodium Potassium ATPase antibody – Plasma Membrane Marker Lane 3 : Anti-ATP5A antibody – Mitochondrial Marker Lane 4 : Anti-GAPDH antibody – Cytosolic Marker Lane 5 : Anti-Histone H3 (di methyl K9) antibody – Nuclear Marker Lane 6 : Assembled Organelle Detection CocktailSecondary: ab131368 at 1/1000 dilution.
All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20. All lanes : Anti-Sodium Potassium ATPase antibody – Plasma Membrane Marker Anti-ATP5A antibody – Mitochondrial Marker Anti-GAPDH antibody – Cytosolic Marker Anti-Histone H3 (di methyl K9) antibody – Nuclear Marker Lane 1 : Marker Lane 2 : Human heart homogenate Whole Tissue Lysate - 20 µg Lane 3 : HeLa Whole Cell Lysate - 20 µg Lane 4 : Mouse heart homogenate Whole Tissue Lysate - 20 µg Lane 5 : NIH-3T3 Whole Cell Lysate - 20 µg Lane 6 : Rat heart homogenate Whole Tissue Lysate - 20 µg Lane 7 : H9C2 Whole Cell Lysate - 20 µg Secondary: ab131368 at 1/1000 dilution.
HeLa cell lysate was prepared using the Cell Fractionation Kit ab109719. All blocking and antibody incubation steps were done in 5% milk, 20 mM Tris-HCl, 0.1% TWEEN-20. All lanes : Anti-Sodium Potassium ATPase antibody – Plasma Membrane Marker Anti-ATP5A antibody – Mitochondrial Marker Anti-GAPDH antibody – Cytosolic Marker Anti-Histone H3 (di methyl K9) antibody – Nuclear Marker Lane 1 : Marker Lane 2 : HeLa Whole Cell Lysate Lane 3 : HeLa Cytosolic Fraction Lysate Lane 4 : HeLa Mitochondrial Fraction Lysate Lane 5 : HeLa Nuclear Fraction Lysate Secondary Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/10000.