Anti-GRP94 antibody

• Supplier: Abcam
• Catalog: ab3674
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Whole protein from dog pancreas
• Description: Rabbit Polyclonal
• Gene: HSP90B1
• Conjugate: Unconjugated

Product images

Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).

Anti-GRP94 antibody (ab3674) at 1/2000 dilution + Cell lysates prepared from mouse fibroblasts

ab3674 at a 1/100 dilution staining human renal cell carcinoma cells by ICC/IF. The cells were permeabilized with 0.2% triton X100 and then blocked with 1%BSA, 4% goat serum prior to incubation with the primary 1antibody for 30 min at 37 °C. Bound antibody was detected using an Alexa fluor ® 488 Goat anti-Rabbit IgG (H+L).See Abreview

Ab3674 (1/1000) staining GPR94 in human stomach using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of peptic and parietal cells of the gastric gland.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

All lanes : Anti-GRP94 antibody (ab3674) at 1/2000 dilutionLane 1 : Whole cell lysate prepared from murine 3T3 cellsLane 2 : Whole cell lysate prepared from murine 3T3 cells, treated with 2.5 ug/ml Tunicamycin 10 hoursLysates/proteins at 50 µg per lane.SecondaryHRP conjugated goat anti-rabbit IgG at 1/2000 dilutiondeveloped using the ECL techniqueObserved band size : 94 kDa (why is the actual band size different from the predicted?)Exposure time : 30 secondsImage courtesy of an anonymous Abreview.See Abreview

Product References

Proteomic analysis of meningiomas. - Proteomic analysis of meningiomas.

Cui GQ, Jiao AH, Xiu CM, Wang YB, Sun P, Zhang LM, Li XG. Acta Neurol Belg. 2014 Sep;114(3):187-94.

Peroxiredoxin-controlled G-CSF signalling at the endoplasmic reticulum-early - Peroxiredoxin-controlled G-CSF signalling at the endoplasmic reticulum-early

Palande K, Roovers O, Gits J, Verwijmeren C, Iuchi Y, Fujii J, Neel BG, Karisch R, Tavernier J, Touw IP. J Cell Sci. 2011 Nov 1;124(Pt 21):3695-705.

HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear - HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear

Lan X, Xu J, Kiyota T, Peng H, Zheng JC, Ikezu T. J Immunol. 2011 Jun 15;186(12):6925-32.

The life span of short-lived plasma cells is partly determined by a block on - The life span of short-lived plasma cells is partly determined by a block on

Auner HW, Beham-Schmid C, Dillon N, Sabbattini P. Blood. 2010 Nov 4;116(18):3445-55.

Evidence for dimeric BACE-mediated APP processing. - Evidence for dimeric BACE-mediated APP processing.

Jin S, Agerman K, Kolmodin K, Gustafsson E, Dahlqvist C, Jureus A, Liu G, Falting J, Berg S, Lundkvist J, Lendahl U. Biochem Biophys Res Commun. 2010 Feb 26;393(1):21-7. doi:

Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein. - Birth and rapid subcellular adaptation of a hominoid-specific CDC14 protein.

Rosso L, Marques AC, Weier M, Lambert N, Lambot MA, Vanderhaeghen P, Kaessmann H. PLoS Biol. 2008 Jun 10;6(6):e140.

Activated epidermal growth factor receptor induces integrin alpha2 - Activated epidermal growth factor receptor induces integrin alpha2

Ning Y, Buranda T, Hudson LG. J Biol Chem. 2007 Mar 2;282(9):6380-7. Epub 2006 Dec 19.