HSP90B1

Gene Symbol HSP90B1
Entrez Gene 7184
Alt Symbol ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1
Species Human
Gene Type protein-coding
Description heat shock protein 90kDa beta (Grp94), member 1
Other Description 94 kDa glucose-regulated protein|endoplasmin|endothelial cell (HBMEC) glycoprotein|epididymis luminal protein 35|epididymis secretory sperm binding protein Li 125m|heat shock protein 90 kDa beta member 1|stress-inducible tumor rejection antigen gp96|tumor rejection antigen (gp96) 1|tumor rejection antigen 1
Swissprots P14625 Q96A97
Accessions AAA58621 AAA68201 CAK18765 EAW97723 EAW97724 P14625 AB209534 BAD92771 AF087988 AJ890084 CAI64497 AK025459 AK025862 AK295262 BAG58251 AK298996 BAG61085 AK300521 BAG62233 AY040226 AAK74072 BC009195 AAH09195 BC066656 AAH66656 CA419931 EU794608 ACJ13662 FJ460515 ACS44652 S74942 AAD14161 X15187 CAA33261 NM_003299 NP_003290
Function Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. {ECO:0000250, ECO:0000269|PubMed:18264092}.
Subcellular Location Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Top Pathways Prostate cancer, NOD-like receptor signaling pathway, Protein processing in endoplasmic reticulum, PI3K-Akt signaling pathway, Estrogen signaling pathway

Grp94 Antibody - 2104 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Grp94 Antibody.

GRP 94 (9G10) - sc-32249 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
goat anti-rat IgG-CFL 405: sc-362253. Immunofluorescence staining of formalin-fixed HepG2 cells showing cytoplasmic localization. Antibody tested: GRP 94 (9G10): sc-32249. Goat anti-rat IgG was conjugated to CruzFluor™ 405 succinimidyl ester: sc-362616.

GRP 94 (H-10) - sc-393402 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GRP 94 (H-10): sc-393402. Western blot analysis of GRP 94 expression in HeLa (A), K-562 (B), KNRK (C), NIH/3T3 (D) and SK-BR-3 (E) whole cell lysates.

GRP 94 (C-19) - sc-1794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GRP 94 (C-19): sc-1794. Western blot analysis of GRP 94 expression in HeLa whole cell lysate.

GRP 94 (H-212) - sc-11402 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GRP 94 (H-212): sc-11402. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.

GRP 94 (SPM249) - sc-56399 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rat
Clonality Monoclonal
GRP 94 (SPM249): sc-56399. Western blot analysis of GRP 94 expression in NIH/3T3 (A), HeLa (B), K-562 (C), KNRK (D) and T-47D (E) whole cell lysates.

GRP 94 (2H3) - sc-53929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
GRP 94 (2H3): sc-53929. Western blot analysis of HSP 90 expression in non-transfected: sc-117752 (A) and human HSP 90 transfected: sc-117081 (B) 293T whole cell lysates.

Anti-GRP94 antibody - ab3674 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunostaining of mouse embryonic fibroblasts with ab3674. (See Nakamura et al. 2001).

Anti-GRP94 antibody - ab18055 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Methanol-fixed HEK293 cells stained at 1/200 with ab18055 followed by FITC conjugated secondary.

Anti-GRP94 antibody - ab53075 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab53075 at 1/50 dilution, with and without immunizing peptide.

Anti-GRP94 antibody - ab104039 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-GRP94 antibody (ab104039) at 1/100 dilution + K562 cell line lysates at 35 µg

Anti-GRP94 antibody - ab87886 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-GRP94 antibody (ab87886) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-GRP94 antibody [2H3] - ab63469 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H3
Anti-GRP94 antibody [2H3] (ab63469) at 1/500 dilution + Human embryonic stem cell lysate at 20 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique

Anti-GRP94 antibody [EPR3988] - ab108606 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3988
All lanes : Anti-GRP94 antibody [EPR3988] (ab108606) at 1/1000 dilutionLane 1 : T47D cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : Jurkat cell lysateLane 5 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-GRP94 antibody [EPR3988] (Alexa Fluor® 488) - ab199042 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3988
ab199042 staining GRP94 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199042 at 1/50 dilution (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed, at 1µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GRP94 antibody [EPR3988] (Alexa Fluor® 647) - ab199206 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3988
ab199206 staining GRP94 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199206 at 1/50 dilution (shown in red) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed, at 1µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-GRP94 antibody, prediluted - ab53903 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Human breast carcinoma stained with neat ab53903

Anti-HSP90B1 / GP96 / GRP94 Antibody - LS-C192314 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of HSP90B1 pAb in extracts from Hela and K562 cells.

Anti-HSP90B1 / GP96 / GRP94 Antibody (clone 9G10) - LS-C202239 from LifeSpan Bioscience

Prices $265.00
Sizes 100 µg
Host Rat
Clonality Monoclonal
Clone 9G10

Anti-HSP90B1 / GP96 / GRP94 Antibody (aa754-803) - LS-C118474 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded human breast carcinoma tissue, using GRP94 Antibody. The picture on the right is treated with the synthesized peptide.

Anti-HSP90B1 / GP96 / GRP94 Antibody - LS-C203200 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-HSP90B1 / GP96 / GRP94 Antibody (C-Terminus) - LS-C209616 from LifeSpan Bioscience

Prices $245.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-HSP90B1 / GP96 / GRP94 Antibody (Glu790) - LS-C176050 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of Endoplasmin (E790) pAb in paraffin-embedded human breast carcinoma tissue.

Anti-HSP90AB1 / HSP90 Alpha B1 Antibody (Glu222) - LS-C176684 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of HSP90B (E222) pAb in extracts from PC12 cells.

Anti-HSP90B1 / GP96 / GRP94 Antibody (aa467-495, AP) - LS-C222756 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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