Anti-Hes1 antibody

• Supplier: Abcam
• Catalog: ab71559
• Prices: $400.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F ICC/IF ICC/IF IHC-P WB
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide derived from the C-terminal domain of (Mouse) Hes1 protein
• Blocking Peptide: Hes1 peptide
• Description: Rabbit Polyclonal
• Gene: HES1
• Conjugate: Unconjugated

Product images

ab71559 staining Hes1 in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone/methanol (1:1), permeabilized with PBS + triton X100 (0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. See Abreview

All lanes : Anti-Hes1 antibody (ab71559)Lane 1 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)Lane 2 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)Lane 3 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)Lane 4 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)Lane 5 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)Lane 6 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)Lane 7 : Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)Lane 8 : Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)

ab71559 staining Hes1 in small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehye and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/200.See Abreview

ab71559 staining Hes1 in the COS1 fibroblast cell line from Monkey kidney by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 21°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.See Abreview

Product References

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