Anti-HIF-1-alpha antibody [ESEE122]
| Name | Anti-HIF-1-alpha antibody [ESEE122] |
|---|---|
| Supplier | Abcam |
| Catalog | ab8366 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | ESEE122 |
| Applications | FC ICC/IF ICC/IF ELISA IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Recombinant fragment corresponding to Human HIF-1-alpha aa 329-530 |
| Description | Mouse Monoclonal |
| Gene | HIF1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Detection of HIF-1-alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using ab8366. 100X magnification. Blue dye is DAPI nuclear staining.Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
Detection of HIF-1-alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using ab8366. Blue dye is DAPI nuclear staining.Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
Detection of HIF-1-alpha (red dye 568) in a cultured raw mouse macrophage cell line, using ab8366. 100X magnification.Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
Detection of HIF-1-alpha (red dye 568) in a cultured raw mouse macrophage cell line, using ab8366.Photos courtesy of Susan Alexander and Hattie Gresham, PhD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of hypoxia-induced Human placenta labeling HIF-1-alpha with ab8366.
![Overlay histogram showing HeLa cells stained with ab8366 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8366, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/570_HIF1-alpha-Primary-antibodies-ab8366-1.jpg)
Overlay histogram showing HeLa cells stained with ab8366 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8366, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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Prognostic significance of hypoxia-inducible factor-1alpha, TWIST1 and Snail - Prognostic significance of hypoxia-inducible factor-1alpha, TWIST1 and Snail
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PGE1 analog alprostadil induces VEGF and eNOS expression in endothelial cells. - PGE1 analog alprostadil induces VEGF and eNOS expression in endothelial cells.
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Expression of hypoxia-inducible factor-1alpha and cell cycle proteins in invasive - Expression of hypoxia-inducible factor-1alpha and cell cycle proteins in invasive
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Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor. - Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor.
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Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor. - Mechanical overload induces VEGF in cartilage discs via hypoxia-inducible factor.
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