HIF1A

Gene Symbol HIF1A
Entrez Gene 281814
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Other Description HIF-1 alpha|HIF-1-alpha|HIF1 alpha|HIF1-alpha|hypoxia-inducible factor 1 alpha|hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|hypoxia-inducible factor 1-alpha
Swissprots Q9XTA5
Accessions DAA25052 Q9XTA5 AB018398 BAA78675 BC126622 AAI26623 NM_174339 NP_776764
Function Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250|UniProtKB:Q61221, ECO:0000255|PROSITE- ProRule:PRU00981}. Note=Colocalizes with HIF3A in the nucleus and speckles. Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. {ECO:0000250|UniProtKB:Q61221}.
Top Pathways Renal cell carcinoma, Central carbon metabolism in cancer, Choline metabolism in cancer, Proteoglycans in cancer, mTOR signaling pathway

Anti-HIF-1-alpha antibody - ab104263 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of HeLa cells labelling HIF-1-alpha with ab104263. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab104263 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1/400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HIF1 alpha immunoreactivity is localized to the cytoplasm and nucleus.

Anti-HIF-1-alpha antibody [ESEE122] - ab8366 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ESEE122
Detection of HIF-1-alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using ab8366. 100X magnification. Blue dye is DAPI nuclear staining.Photos courtesy of Susan Alexander and Hattie Gresham, PhD.

Anti-HIF-1-alpha antibody [ESEE122] (FITC) - ab187786 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122

Anti-HIF-1-alpha antibody [H1alpha67] - ChIP Grade - ab1 from Abcam

Prices $403.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone H1alpha67
ab1 at 1/200 dilution staining HIF-1-alpha in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized in 0.5% Trition X-100 and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/500 dilution.See Abreview

Anti-HIF-1-alpha antibody [H1alpha67] - ChIP Grade - ab463 from Abcam

Prices $401.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling HIF-1-alpha with ab463.

Anti-HIF-1-alpha antibody [mgc3] - ab16066 from Abcam

Prices $404.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mgc3
Immunocytochemistry/Immunofluorescent analysis of HIF-1-alpha (green) in HeLa cells either left untreated (left panel) or treated with 100uM Deferoxamine mesylate for ~16 hours (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with ab16066 at a dilution of 1/100 for at least 1 hour at room temperature and washed with PBS. Cells were then incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1/500 for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin and nuclei (blue) were stained with Hoechst dye.

Anti-HIF-1-alpha antibody - ChIP Grade - ab2185 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab2185 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

HIF1a Antibody - abx103444 from Abbexa

Host Rabbit
Clonality Polyclonal

Anti-Hypoxia Inducible Factor 1 Alpha (HIF1a) - 20-3184 from ARP American Research Products

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

HIF1A Monoclonal Antibody (ESEE122) - MA1-16518 from Thermo Fisher Scientific

Prices $354.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122
<h6 id="pdp-product-imgtitle"> HIF1A Antibody (MA1-16518) </h6><p id="pdp-product-imgdesc"> Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using MA1-16518. 100X magnification. Blue dye is DAPI nuclear staining. </p>

HIF1A Monoclonal Antibody (ESEE122), Biotin - MA5-16016 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122
HIF-1 alpha Antibody (MA5-16016) in WB

HIF1A Monoclonal Antibody (ESEE122), DyLight 650 - MA5-16017 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122

HIF1A Monoclonal Antibody (ESEE122), DyLight 488 - MA5-16018 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122

HIF1A Monoclonal Antibody (ESEE122), HRP - MA5-16019 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122
HIF-1 alpha Antibody (MA5-16019) in IHC

HIF1A Monoclonal Antibody (ESEE122), DyLight 550 - MA5-16020 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ESEE122

HIF1A Monoclonal Antibody (H1alpha67) - MA1-16504 from Thermo Fisher Scientific

Prices $354.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67
<h6 id="pdp-product-imgtitle"> HIF1A Antibody (MA1-16504) </h6><p id="pdp-product-imgdesc"> Immunohistochemical staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining. </p>

HIF1A Monoclonal Antibody (H1alpha67) - MA1-16511 from Thermo Fisher Scientific

Prices $354.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67
<h6 id="pdp-product-imgtitle"> HIF1A Antibody (MA1-16511) </h6><p id="pdp-product-imgdesc"> Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-16511. </p>

HIF1A Monoclonal Antibody (H1alpha67), Biotin - MA1-46457 from Thermo Fisher Scientific

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67
On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.

HIF1A Monoclonal Antibody (H1alpha67), DyLight 650 - MA5-16008 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67

HIF1A Monoclonal Antibody (H1alpha67), DyLight 488 - MA5-16009 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67

HIF1A Monoclonal Antibody (H1alpha67), DyLight 550 - MA5-16010 from Thermo Fisher Scientific

Prices $375.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone H1alpha67

HIF1A Monoclonal Antibody (mgc3) - MA1-516 from Thermo Fisher Scientific

Prices $363.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone mgc3
<h6 id="pdp-product-imgtitle"> HIF1A Antibody (MA1-516) in IF </h6><p id="pdp-product-imgdesc"> Immunofluorescent analysis of HIF-1 alpha (green) in HeLa cells either left untreated (left panel) or treated with 100uM Deferoxamine mesylate for ~16 hours (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a HIF-1 alpha monoclonal antibody (Product # MA1-516) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:500 for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin (Product # 21836) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification. </p>

HIF-1 alpha Antibody [ESEE122] - IQ607 from ImmunQuest

Host Mouse
Clone ESEE122

HIF-1 alpha Antibody [ESEE122]: Fluorescein - IQ607FITC from ImmunQuest

Host Mouse
Clone ESEE122

HIF1 alpha antibody - 70R-51558 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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