Anti-HIF-1-alpha antibody - ChIP Grade
| Name | Anti-HIF-1-alpha antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab2185 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IP EMSA IHC-F ICC/IF ICC/IF ICC/IF ChIP |
| Species Reactivities | Mouse, Rat, Guinea Pig, Human, Xenopus, Monkey, Bovine |
| Antigen | Fusion protein corresponding to Human HIF-1-alpha aa 432-528 |
| Description | Rabbit Polyclonal |
| Gene | HIF1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab2185 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling HIF-1-alpha with ab2185.
All lanes : Anti-HIF-1-alpha antibody - ChIP Grade (ab2185)Lane 1 : Rat nuclear extract lysate - normoxicLane 2 : Rat nuclear extract lysate - hypoxic
ChIP analysis of HIF-1-alpha genomic sequences from HeLa cells cultivated in normoxic (N) or hypoxic (Hx) conditions, using a HIF1-alpha polyclonal antibody (ab2185). For a negative control, IgG was used and the input as a positive control in the subsequent PCR. Primers for known target genes were used HIF1 alpha, A. EPO and B. VEGF.
Product References
Hypoxia-induced and calpain-dependent cleavage of filamin A regulates the hypoxic - Hypoxia-induced and calpain-dependent cleavage of filamin A regulates the hypoxic
Zheng X, Zhou AX, Rouhi P, Uramoto H, Boren J, Cao Y, Pereira T, Akyurek LM, Poellinger L. Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2560-5. doi:
Fine-mapping quantitative trait loci affecting murine external ear tissue - Fine-mapping quantitative trait loci affecting murine external ear tissue
Cheverud JM, Lawson HA, Bouckaert K, Kossenkov AV, Showe LC, Cort L, Blankenhorn EP, Bedelbaeva K, Gourevitch D, Zhang Y, Heber-Katz E. Heredity (Edinb). 2014 May;112(5):508-18.
Regulation of CDKN2B expression by interaction of Arnt with Miz-1--a basis for - Regulation of CDKN2B expression by interaction of Arnt with Miz-1--a basis for
Aesoy R, Gradin K, Aasrud KS, Hoivik EA, Ruas JL, Poellinger L, Bakke M. Mol Cancer. 2014 Mar 11;13:54.
A novel distal upstream hypoxia response element regulating oxygen-dependent - A novel distal upstream hypoxia response element regulating oxygen-dependent
Storti F, Santambrogio S, Crowther LM, Otto T, Abreu-Rodriguez I, Kaufmann M, Hu CJ, Dame C, Fandrey J, Wenger RH, Hoogewijs D. Haematologica. 2014 Apr;99(4):e45-8.
Hypoxia promotes uveal melanoma invasion through enhanced Notch and MAPK - Hypoxia promotes uveal melanoma invasion through enhanced Notch and MAPK
Asnaghi L, Lin MH, Lim KS, Lim KJ, Tripathy A, Wendeborn M, Merbs SL, Handa JT, Sodhi A, Bar EE, Eberhart CG. PLoS One. 2014 Aug 28;9(8):e105372.
Hypoxia-inducible factor-1alpha induces ErbB4 signaling in the differentiating - Hypoxia-inducible factor-1alpha induces ErbB4 signaling in the differentiating
Paatero I, Seagroves TN, Vaparanta K, Han W, Jones FE, Johnson RS, Elenius K. J Biol Chem. 2014 Aug 8;289(32):22459-69.
Insulin regulates hypoxia-inducible factor-1alpha transcription by reactive - Insulin regulates hypoxia-inducible factor-1alpha transcription by reactive
Biswas S, Mukherjee R, Tapryal N, Singh AK, Mukhopadhyay CK. PLoS One. 2013 Apr 23;8(4):e62128.
Hypoxia-inducible factor-1alpha (HIF-1alpha) promotes cap-dependent translation - Hypoxia-inducible factor-1alpha (HIF-1alpha) promotes cap-dependent translation
Yi T, Papadopoulos E, Hagner PR, Wagner G. J Biol Chem. 2013 Jun 28;288(26):18732-42.
Hypoxic retinal Muller cells promote vascular permeability by HIF-1-dependent - Hypoxic retinal Muller cells promote vascular permeability by HIF-1-dependent
Xin X, Rodrigues M, Umapathi M, Kashiwabuchi F, Ma T, Babapoor-Farrokhran S, Wang S, Hu J, Bhutto I, Welsbie DS, Duh EJ, Handa JT, Eberhart CG, Lutty G, Semenza GL, Montaner S, Sodhi A. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3425-34. doi:
Hypoxia-inducible factor 1-induced G protein-coupled receptor 35 expression is an - Hypoxia-inducible factor 1-induced G protein-coupled receptor 35 expression is an
Ronkainen VP, Tuomainen T, Huusko J, Laidinen S, Malinen M, Palvimo JJ, Yla-Herttuala S, Vuolteenaho O, Tavi P. Cardiovasc Res. 2014 Jan 1;101(1):69-77.