HIF1A Monoclonal Antibody (H1alpha67), Biotin

Name	HIF1A Monoclonal Antibody (H1alpha67), Biotin
Supplier	Thermo Fisher Scientific
Catalog	MA1-46457
Prices	$325.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	H1alpha67
Applications	IHC-P IP WB
Species Reactivities	Bird, Bovine, Dog, Ferret, Human, Mouse, Primate, Sheep, Pig, Rat
Antigen	Fusion protein containing amino acids 432-528 of human HIF-1alpha
Purity/Format	Protein A
Description	Mouse Monoclonal
Gene	HIF1A
Conjugate	Biotin
Supplier Page	Shop

Product images

$On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.$ On day 1, MEF cells (+/+,-/-), were grown on 15cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution. The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution.

Immunoprecipitation of HIF-1alpha using MA1-46457; Heavy and light chains are also detected.

Detection of HIF-1 alpha in cobalt chloride treated/untreated COS-7 nuclear extracts using MA1-46457.

Immunohistochemical staining of HIF-1 alpha in human kidney using MA1-46457.