HIF1A Monoclonal Antibody (mgc3)
| Name | HIF1A Monoclonal Antibody (mgc3) |
|---|---|
| Supplier | Thermo Fisher Scientific |
| Catalog | MA1-516 |
| Prices | $363.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | mgc3 |
| Applications | EMSA ICC/IF Immunoelectrophoresis IHC-P IP WB |
| Species Reactivities | Bovine, Human, Mouse, Primate, Pig |
| Antigen | Human HIF-1 alpha amino acids 530-826. |
| Purity/Format | Protein A |
| Description | Mouse Monoclonal |
| Gene | HIF1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
HIF1A Antibody (MA1-516) in IF
Immunofluorescent analysis of HIF-1 alpha (green) in HeLa cells either left untreated (left panel) or treated with 100uM Deferoxamine mesylate for ~16 hours (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a HIF-1 alpha monoclonal antibody (Product # MA1-516) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:500 for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin (Product # 21836) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification.