Anti-HIF-2-alpha antibody
| Name | Anti-HIF-2-alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab20654 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide corresponding to Mouse HIF-2-alpha aa 600-700 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Mouse HIF-2-alpha peptide |
| Description | Rabbit Polyclonal |
| Gene | EPAS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab20654 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab20654, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Image courtesy of Human Protein Atlasab20654 staining HIF-2-alpha in Adrenal gland. The paraffin embedded human tissue was incubated with ab20654(1:2500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. Ab20654 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.Further results for this antibody can be found at www.proteinatlas.org
All lanes : Anti-HIF-2-alpha antibody (ab20654) at 1 µg/mlLane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell LysateLane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Nuclear Lysate - hypoxia treated (ab14886)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Preconditioning of mesenchymal stem cells by sevoflurane to improve their - Preconditioning of mesenchymal stem cells by sevoflurane to improve their
Sun X, Fang B, Zhao X, Zhang G, Ma H. PLoS One. 2014 Mar 5;9(3):e90667.
Sub-sets of cancer stem cells differ intrinsically in their patterns of oxygen - Sub-sets of cancer stem cells differ intrinsically in their patterns of oxygen
Gammon L, Biddle A, Heywood HK, Johannessen AC, Mackenzie IC. PLoS One. 2013 Apr 30;8(4):e62493.
Assessment of hypoxia inducible factor levels in cancer cell lines upon hypoxic - Assessment of hypoxia inducible factor levels in cancer cell lines upon hypoxic
Zhou W, Dosey TL, Biechele T, Moon RT, Horwitz MS, Ruohola-Baker H. PLoS One. 2011;6(11):e27460.
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