EPAS1

Gene Symbol EPAS1
Entrez Gene 2034
Alt Symbol ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
Species Human
Gene Type protein-coding
Description endothelial PAS domain protein 1
Other Description EPAS-1|HIF-1-alpha-like factor|HIF-1alpha-like factor|HIF-2-alpha|HIF2-alpha|PAS domain-containing protein 2|basic-helix-loop-helix-PAS protein MOP2|class E basic helix-loop-helix protein 73|endothelial PAS domain-containing protein 1|hypoxia-inducible factor 2 alpha|hypoxia-inducible factor 2-alpha|member of PAS protein 2
Swissprots Q99630 Q86VA2 Q99814
Accessions AAX93237 AAY14704 EAX00255 Q99814 AF052094 AK123845 BAG53969 BC015869 BC051338 AAH51338 BM671511 CD109908 U51626 AAC51212 U81984 AAB41495 XM_011532698 XP_011531000 NM_001430 NP_001421
Function Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Subcellular Location Nucleus {ECO:0000250|UniProtKB:P97481, ECO:0000255|PROSITE-ProRule:PRU00981}. Nucleus speckle {ECO:0000250|UniProtKB:P97481}. Note=Colocalizes with HIF3A in the nucleus and speckles. {ECO:0000250|UniProtKB:P97481}.
Tissue Specificity Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Top Pathways Renal cell carcinoma

HIF-2α (D9E3) Rabbit mAb - 7096 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9E3
Western blot analysis of Hep G2 cells, untreated (-) or treated with cobalt chloride (CoCl 2 , 0.1 mM, 4 hr, +) using HIF-2α (D9E3) Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).

EPAS-1 (A-5) - sc-46691 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EPAS-1 (A-5): sc-46691. Western blot analysis of EPAS-1 expression in untreated (A) and CoCl<sub>2</sub>-treated (B) HeLa whole cell lysates.

EPAS-1 (190b) - sc-13596 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
EPAS-1 (190b): sc-13596. Western blot analysis of EPAS-1 expression in untreated (A) and CoCl<sub>2</sub>-induced (B) HeLa whole cell lysate.

EPAS-1 (C-16) - sc-8712 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

EPAS-1 (H-310) - sc-28706 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
EPAS-1 (H-310): sc-28706. Western blot analysis of EPAS-1 expression in HT-1080 (A), CoCl<sub>2</sub>-induced HeLa (B) and A549 (C) whole cell lysates.

EPAS-1 (K-20) - sc-32146 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
EPAS-1 (K-20): sc-32146. Western blot analysis of EPAS-1 expression in H4 whole cell lysate.

EPAS-1 (R-17) - sc-32147 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-HIF-2-alpha antibody - ab20654 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab20654 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab20654, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). 

Anti-HIF-2-alpha antibody - ab109616 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab109616 staining Hif-2-alpha in HeLa cells. The cells were incubated with 1mM Deferoxamine for 24 hours (Treated) or solvent-only for control purposes (Solvent only). Cells were fixed with 4% formaldehyde (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab109616 at 10µg/ml and ab195889 at 1µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI. 

Anti-HIF-2-alpha antibody [2G5] - ab157249 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G5
All lanes : Anti-HIF-2-alpha antibody [2G5] (ab157249) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY HIF2 alphaLysates/proteins at 5 µg per lane.

Anti-HIF-2-alpha antibody [ep190b] - ab8365 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ep190b
Immunohistochemical analysis of Human breast cancer tissue, staining HIF-2-alpha with ab8365. Antigen retrieval was performed using EDTA buffer in a pressure cooker, before being blocked with normal serum. Samples were incubated with primary antibody (1/2000) overnight at 4°C. Staining was detected using DAB.

Anti-HIF-2-alpha antibody [ep190b] (Biotin) - ab72715 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ep190b

Anti-HIF-2-alpha antibody [ep190b] (HRP) - ab81805 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ep190b
All lanes : anti HIF2 alpha (unconjugated form of ab81805) at 1/500 dilutionLane 1 : normoxic A549 human lysate controlLane 2 : hypoxic A549 human lysate

Anti-HIF-2-alpha antibody [EP5876(2)] - ab179825 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP5876(2)
Flow cytometric analysis of permeabilized HeLa cells labeling HIF2 alpha using ab179825 at 1/10 dilution (red) or a rabbit IgG negative (green).

Anti-HIF-2-alpha antibody - ChIP Grade - ab199 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab199 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab199, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HIF2A / EPAS1 Antibody (C-Terminus, DY650) - LS-C140799 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF2A / EPAS1 Antibody (aa535-631, clone ep190b, HRP) - LS-C83709 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone ep190b
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.

Anti-HIF2A / EPAS1 Antibody (C-Terminus, DY488) - LS-C140800 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF2A / EPAS1 Antibody (C-Terminus, DY550) - LS-C140801 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIF2A / EPAS1 Antibody (aa535-631, clone Ep190b, DY650) - LS-C140826 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone Ep190b

Anti-HIF2A / EPAS1 Antibody (aa535-631, clone Ep190b, DY550) - LS-C140828 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone Ep190b

Anti-HIF2A / EPAS1 Antibody (aa360-409) - LS-C109647 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EPAS1 / HIF2A antibody LS-C109647 Western blot of 293T cell lysate.

Anti-HIF2A / EPAS1 Antibody (aa542-874) - LS-C143898 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-HIF2A / EPAS1 Antibody (aa535-631, clone Ep190b, DY488) - LS-C140827 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone Ep190b

Anti-HIF2A / EPAS1 Antibody (aa535-631, clone Ep190b, Biotin) - LS-C140825 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone Ep190b
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.
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