Anti-HIF-2-alpha antibody - ChIP Grade
| Name | Anti-HIF-2-alpha antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab199 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP IP ICC/IF ICC/IF IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to Mouse HIF-2-alpha aa 632-646 |
| Description | Rabbit Polyclonal |
| Gene | EPAS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab199 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab199, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab199 staining human adrenal tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 10 minutes at 25°C. The primary anitbody was diluted 1/500 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody was used as the secondary.See Abreview
All lanes : Anti-HIF-2-alpha antibody - ChIP Grade (ab199) at 1/500 dilutionLane 1 : 20ug of whole cell lysate from PC12 cells.Lane 2 : 20ug of whole cell lysate from PC12 cells exposed to hypoxic conditions (12 hours at 3% oxygen).Lane 3 : 20ug of whole cell lysate from PC12 cells exposed to hypoxic conditions (12 hours at 3% oxygen) and incubated with antisense HIF2 alpha oligonucleotides 72 hours prior to treatment.developed using the ECL techniquePerformed under reducing conditions.
Product References
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