Anti-Histone H2A (acetyl K5) antibody [EP856Y]

• Supplier: Abcam
• Catalog: ab45152
• Prices: $397.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP856Y
• Applications: WB IHC-P ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human, C. elegans
• Antigen: Synthetic peptide corresponding to residues near the N-terminus of human Histone H2A
• Description: Rabbit Monoclonal
• Gene: CELE_ZK1251.1
• Conjugate: Unconjugated

Product images

IHC - Wholemount of Caenorhabditis elegans embryo labelling Histone H2A (acetyl K5) with ab45152. Sample was incubated with primary antibody (1/500 in 3% BSA + 0.1% Triton in TBS buffer) for 12 hours at 4°C. An Alexa Flour® 546-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Top - Telomere (green), Middle - Histone H2A (acetyl K5) (red), Bottom - merge.See Abreview

Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/1000 dilution + Mouse cortex whole tissue lysate at 20 µgSecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] (ab45152) at 1/100000 dilutionLane 1 : C6 cell lysateLane 2 : C6 + TSA membrane

Ab45152 staining human Histone H2A in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.Ab45152 staining human Histone H2A in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.

ICC/IF image of ab45152 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45152, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab45152 staining Histone H2A (acetyl K5) in HeLa cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/300) for 1 hour at 20°C. An Allophycocyanin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.Gating Strategy: Isotype population (shown in white).See Abreview

Product References

Histone acetyltransferase 1 promotes homologous recombination in DNA repair by - Histone acetyltransferase 1 promotes homologous recombination in DNA repair by

Yang X, Li L, Liang J, Shi L, Yang J, Yi X, Zhang D, Han X, Yu N, Shang Y. J Biol Chem. 2013 Jun 21;288(25):18271-82.