CELE_ZK1251.1

Gene Symbol CELE_ZK1251.1
Entrez Gene 191549
Alt Symbol -
Species C. elegans
Gene Type protein-coding
Description HTAS-1
Other Description -
Accessions CAA92497 Q23429 NM_069317 NP_501718

Anti-Histone H2A (acetyl K5) antibody [EP856Y] - ab45152 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP856Y
IHC - Wholemount of Caenorhabditis elegans embryo labelling Histone H2A (acetyl K5) with ab45152. Sample was incubated with primary antibody (1/500 in 3% BSA + 0.1% Triton in TBS buffer) for 12 hours at 4°C. An Alexa Flour® 546-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Top - Telomere (green), Middle - Histone H2A (acetyl K5) (red), Bottom - merge.See Abreview

Anti-Histone H2A (acetyl K5) antibody - ChIP Grade - ab1764 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Histone H2A antibody - ChIP Grade - ab88770 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab88770 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Anti-Histone H2A antibody - ChIP Grade - ab13923 from Abcam

Prices $403.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab13923 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13923, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ZK1251.1 (aa 1-5/81-96) polyclonal antibody - CABT-BL3847 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal
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