Anti-Histone H2A (acetyl K5) antibody - ChIP Grade
| Name | Anti-Histone H2A (acetyl K5) antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab1764 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ChIP ELISA IP WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, C. elegans, Drosophila, Mammalian |
| Antigen | Synthetic peptide: SGRGKAcQGGKYC conjugated to Ovalbumin |
| Description | Rabbit Polyclonal |
| Gene | CELE_ZK1251.1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of ab1764 staining Histone H2A (acetyl K5) in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1764, 1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC - Wholemount of Caenorhabditis elegans whole body labelling Histone H2A (acetyl K5) with ab1764. Sample was incubated with primary antibody (1/2500 in 3% BSA + 0.1% Triton X-100 in PBS) for 12 hours at 4°C. An Alexa Flour® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Left - Histone H2A (acetyl K5) (green), Right - nuclei (DAPI, blue). See Abreview
ab1764 staining Female Embryonic Stem cells differentiated for 7 days by immunocytochemistry. The antibody was used at a dilution of 1/200 and incubated with the cells for 1 hour. Bound antibody was detected with a FITC conjugated Goat anti-Rabbit polyclonal antibody. DAPI counterstain is shown pseudocoloured as white on the right of the image (b). Centric heterochromatin was underacetylated in differentiated cells (arrowhead in a). A pale staining chromosome was observed at high frequencies; previoulsy shown to be the inactive female X chromosome (arrow in a).This image is courtesy of an Abreview by Hugh Spotswood submitted on 13 February 2006.See Abreview
Observed band size : 15 kDa (why is the actual band size different from the predicted?)This image is courtesy of an anonymous AbreviewAb1764 at a 1/1000 dilution in Western blot of human Osteosarcoma (U2OS) nuclear cell lysate.See Abreview
ab1764 staining Histone H2A (acetyl K5) in HeLa cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Gating Strategy: Isotype population (shown in white).See Abreview
Product References
SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin. - SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin.
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Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit DNA-repair - Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit DNA-repair
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Np95 is implicated in pericentromeric heterochromatin replication and in major - Np95 is implicated in pericentromeric heterochromatin replication and in major
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Myc-binding-site recognition in the human genome is determined by chromatin - Myc-binding-site recognition in the human genome is determined by chromatin
Guccione E, Martinato F, Finocchiaro G, Luzi L, Tizzoni L, Dall' Olio V, Zardo G, Nervi C, Bernard L, Amati B. Nat Cell Biol. 2006 Jul;8(7):764-70. Epub 2006 Jun 11.
Transcription-induced chromatin remodeling at the c-myc gene involves the local - Transcription-induced chromatin remodeling at the c-myc gene involves the local
Farris SD, Rubio ED, Moon JJ, Gombert WM, Nelson BH, Krumm A. J Biol Chem. 2005 Jul 1;280(26):25298-303. Epub 2005 May 6.